Parvovirus B19 NS1 protein induces synovitis mimicking rheumatoid arthritis

• Published in: Scientific reports Vol. 15; no. 1; pp. 28679 - 11
• Main Authors: Yen, Chang-Yi, Tseng, Chia-Chun, Lin, Chia‐Hui, Lin, Yuan‐Zhao, Li, Ruei‐Nian, Wang, Pin-Yi, Chen, Kuan-Yu, Wu, Cheng-Chin, Ou, Tsan-Teng, Chen, Chung-Jen, Liu, Yu-Peng, Yen, Jeng-Hsien
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 06.08.2025; Nature Publishing Group; Nature Portfolio
• Subjects: 692/4023; 692/699/1670/498; Apoptosis; Arthritis, Rheumatoid - etiology; Arthritis, Rheumatoid - metabolism; Arthritis, Rheumatoid - pathology; Arthritis, Rheumatoid - virology; Autoimmune diseases; Cell Line; Cellular stress response; Cytokines; Datasets; Enzymes; Growth factors; Humanities and Social Sciences; Humans; Infections; Inflammation; Kinases; Mimicry; multidisciplinary; NS1 protein; Osteoarthritis; Oxidative stress; Parvoviridae Infections - complications; Parvoviridae Infections - virology; Parvovirus B19; Parvovirus B19, Human - genetics; Parvovirus B19, Human - metabolism; Parvoviruses; Pathogenesis; Proteins; Rheumatoid arthritis; RNA-seq; Science; Science (multidisciplinary); Signal Transduction; Standard scores; Synovitis; Synovitis - etiology; Synovitis - metabolism; Synovitis - pathology; Synovitis - virology; Synovium; TOR protein; Viral infections; Viral Nonstructural Proteins - genetics; Viral Nonstructural Proteins - metabolism; Synovitis; RNA-seq; Rheumatoid arthritis; Parvovirus B19

This study aims to investigate the role of parvovirus B19 infection in the pathogenesis of rheumatoid arthritis (RA). B19-NS1 DNA was cloned into the LentiORF pLEX-MCS vector, then co-transfected with the pLEX-MCS-NS1 vector and packaging system to produce LEX-NS1 lentiviral stock. LEX was also prep...