Parvovirus B19 NS1 protein induces synovitis mimicking rheumatoid arthritis

• Published in: Scientific reports Vol. 15; no. 1; pp. 28679 - 11
• Main Authors: Yen, Chang-Yi, Tseng, Chia-Chun, Lin, Chia‐Hui, Lin, Yuan‐Zhao, Li, Ruei‐Nian, Wang, Pin-Yi, Chen, Kuan-Yu, Wu, Cheng-Chin, Ou, Tsan-Teng, Chen, Chung-Jen, Liu, Yu-Peng, Yen, Jeng-Hsien
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 06.08.2025; Nature Publishing Group; Nature Portfolio
• Subjects: 692/4023; 692/699/1670/498; Apoptosis; Arthritis, Rheumatoid - etiology; Arthritis, Rheumatoid - metabolism; Arthritis, Rheumatoid - pathology; Arthritis, Rheumatoid - virology; Autoimmune diseases; Cell Line; Cellular stress response; Cytokines; Datasets; Enzymes; Growth factors; Humanities and Social Sciences; Humans; Infections; Inflammation; Kinases; Mimicry; multidisciplinary; NS1 protein; Osteoarthritis; Oxidative stress; Parvoviridae Infections - complications; Parvoviridae Infections - virology; Parvovirus B19; Parvovirus B19, Human - genetics; Parvovirus B19, Human - metabolism; Parvoviruses; Pathogenesis; Proteins; Rheumatoid arthritis; RNA-seq; Science; Science (multidisciplinary); Signal Transduction; Standard scores; Synovitis; Synovitis - etiology; Synovitis - metabolism; Synovitis - pathology; Synovitis - virology; Synovium; TOR protein; Viral infections; Viral Nonstructural Proteins - genetics; Viral Nonstructural Proteins - metabolism; Synovitis; RNA-seq; Rheumatoid arthritis; Parvovirus B19
• DOI: 10.1038/s41598-025-11900-3
• ISSN: 2045-2322

This study aims to investigate the role of parvovirus B19 infection in the pathogenesis of rheumatoid arthritis (RA). B19-NS1 DNA was cloned into the LentiORF pLEX-MCS vector, then co-transfected with the pLEX-MCS-NS1 vector and packaging system to produce LEX-NS1 lentiviral stock. LEX was also prepared as the control. Transduction of LEX-NS1 or LEX lentivirus into the SW982 cell line then proceeded, and total RNA was extracted for RNA-seq. Pathway analysis and comparison of pathway status between this study and GEO datasets were performed, demonstrating that acute phase response signaling, NRF2-mediated oxidative stress response, and mTOR signaling pathways were activated after the transduction of LEX-NS1 into the SW982 cell line, although the interferon signaling pathway was inhibited. The comparison of all pathways using hierarchical clustering showed that the pathway status after transduction of B19-NS1 was closer to RA than that in osteoarthritis. Similar findings can also be found in cellular immune, humoral immune, cytokine, apoptosis, cellular growth, cellular stress, growth factor, and intracellular pathways. In conclusion, parvovirus B19-NS1 can induce inflammation of the synovium mimicking synovitis in RA; therefore, parvovirus B19 infection might be one of the trigger factors in the pathogenesis of RA.