Anti-protein arginine methyltransferase 5 (PRMT5) antibodies is associated with interstitial lung disease in rheumatoid arthritis

• Published in: Scientific reports Vol. 15; no. 1; pp. 29421 - 7
• Main Authors: Huang, Renliang, Guo, Jiaxi, Zhou, Qiaomiao, Huang, Heqing, Lin, Shaowei, Lin, Yikai, Petersen, Frank, Yu, Xinhua, Ma, Aiping
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 11.08.2025; Nature Publishing Group; Nature Portfolio
• Subjects: 631/250; 692/4023; Adult; Aged; Anti-PRMT5 antibodies; Antibodies; Antigens; Antinuclear antibodies; Arthritis, Rheumatoid - blood; Arthritis, Rheumatoid - complications; Arthritis, Rheumatoid - immunology; Autoantibodies - blood; Autoantibodies - immunology; Autoimmune diseases; Biomarkers; Biomarkers - blood; Case-Control Studies; Enzyme-linked immunosorbent assay; Expected values; Female; Humanities and Social Sciences; Humans; Interstitial lung diseases; Investigations; Lung diseases; Lung Diseases, Interstitial - blood; Lung Diseases, Interstitial - complications; Lung Diseases, Interstitial - immunology; Male; Middle Aged; multidisciplinary; Protein arginine methyltransferase; Protein-Arginine N-Methyltransferases - immunology; Proteins; Rheumatoid arthritis; Rheumatology; ROC Curve; Science; Science (multidisciplinary); Scleroderma; Scleroderma, Systemic - blood; Scleroderma, Systemic - immunology; Serum levels; Software; Systemic sclerosis; Variance analysis; China; Anti-PRMT5 antibodies; Interstitial lung diseases; Rheumatoid arthritis; Systemic sclerosis
• DOI: 10.1038/s41598-025-14741-2
• ISSN: 2045-2322

Recently, anti-protein arginine methyltransferase 5 (PRMT5) antibodies has been identified a novel marker for systemic sclerosis (SSc). The present study aimed to to explore the association between anti-PRMT5 antibodies and rheumatoid arthritis (RA). The study recruited a cohort of 33 patients diagnosed with SSc, 87 patients with RA, and 31 healthy control subjects. Serum levels of the anti-PRMT5 antibodies were measured using a double-antigen sandwich enzyme-linked immunosorbent assay (ELISA). Patients with SSc exhibited higher serum levels ( p  < 0.001) and seropositivity rates (48.5% vs. 0%, p  < 0.0001) of anti-PRMT5 antibodies compared to healthy subjects. Notably, serum levels of anti-PRMT5 antibodies were significantly elevated in patients with RA compared to healthy controls ( p  < 0.001), with a corresponding increase in the rate of seropositivity (14.9% vs. 0%, p  < 0.05). Receiver operating curve analysis for anti-PRMT5 antibody levels yielded area under the curve values of 0.768 for distinguishing RA patients from healthy controls, and 0.903 for distinguishing SSc patients from controls. Furthermore, elevated levels of anti-PRMT5 antibodies were significantly associated with the presence of interstitial lung disease (ILD) ( p  < 0.0001) and antinuclear antibody positivity ( p  < 0.01). This study not only confirms the association of the anti-PRMT5 antibodies with SSc but also, for the first time, demonstrates a significant association between the anti-PRMT5 antibodies and RA, particularly in the context of RA-associated ILD.