Detection of Fetal NRBCs in Maternal Blood of Pregnant Carriers of β-Thalassemia Using Anti-γ and Anti-ε Monoclonal Antibodies
: Fetal nucleated red blood cells (NRBCs) entering maternal circulation during pregnancy constitute a potential source of material for safe and reliable noninvasive prenatal diagnosis. The increased prevalence of β‐thalassemia mutations in countries like Greece may create a problem, making it diffic...
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Published in | Annals of the New York Academy of Sciences Vol. 945; no. 1; pp. 151 - 152 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
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Oxford, UK
Blackwell Publishing Ltd
01.09.2001
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Abstract | : Fetal nucleated red blood cells (NRBCs) entering maternal circulation during pregnancy constitute a potential source of material for safe and reliable noninvasive prenatal diagnosis. The increased prevalence of β‐thalassemia mutations in countries like Greece may create a problem, making it difficult to distinguish between NRBCs of fetal or maternal origin. Use of Ab against embryonic hemoglobin ε may increase specificity for fetal NRBC detection. In the present study, Ab against embryonic hemoglobin ε was used in the first and second trimesters of pregnancy in order to determine if specificity for fetal NRBC detection could be increased. |
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AbstractList | Fetal nucleated red blood cells (NRBCs) entering maternal circulation during pregnancy constitute a potential source of material for safe and reliable noninvasive prenatal diagnosis. The increased prevalence of beta-thalassemia mutations in countries like Greece may create a problem, making it difficult to distinguish between NRBCs of fetal or maternal origin. Use of Ab against embryonic hemoglobin epsilon may increase specificity for fetal NRBC detection. In the present study, Ab against embryonic hemoglobin epsilon was used in the first and second trimesters of pregnancy in order to determine if specificity for fetal NRBC detection could be increased. : Fetal nucleated red blood cells (NRBCs) entering maternal circulation during pregnancy constitute a potential source of material for safe and reliable noninvasive prenatal diagnosis. The increased prevalence of β‐thalassemia mutations in countries like Greece may create a problem, making it difficult to distinguish between NRBCs of fetal or maternal origin. Use of Ab against embryonic hemoglobin ε may increase specificity for fetal NRBC detection. In the present study, Ab against embryonic hemoglobin ε was used in the first and second trimesters of pregnancy in order to determine if specificity for fetal NRBC detection could be increased. A bstract : Fetal nucleated red blood cells (NRBCs) entering maternal circulation during pregnancy constitute a potential source of material for safe and reliable noninvasive prenatal diagnosis. The increased prevalence of β‐thalassemia mutations in countries like Greece may create a problem, making it difficult to distinguish between NRBCs of fetal or maternal origin. Use of Ab against embryonic hemoglobin ε may increase specificity for fetal NRBC detection. In the present study, Ab against embryonic hemoglobin ε was used in the first and second trimesters of pregnancy in order to determine if specificity for fetal NRBC detection could be increased. |
Author | MAVROU, ARIADNI KORATZIS, ASTERIS KOLIALEXI, AGGELIKI METAXOTOU, CATHERINE ANTSAKLIS, ARISTIDES KRIKOS, XENOFON |
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References | Mavrou, A., A. Kolialexi et al. 1999. Improved specificity on NRBC detection in chorionic villus sample supernatant fluids using anti-zeta and anti-epsilon monoclonal antibodies. Fetal Diagn. Ther. 14: 291-295. Slunga-Talberg, A., W. El-Rifai et al. 1995. Maternal origin of erythrocytes in peripheral venous blood of pregnant women. Hum. Genet. 96: 53-57. Zheng, Y.L., N.P. Carter et al. 1993. Prenatal diagnosis from maternal blood: simultaneous immunophenotyping and FISH of fetal nucleated erythrocytes isolated by negative magnetic cell sorting. J. Med. Genet. 30: 1051-1056. 1995; 96 1993; 30 1999; 14 e_1_2_5_3_2 e_1_2_5_2_2 e_1_2_5_4_2 |
References_xml | – volume: 96 start-page: 53 year: 1995 end-page: 57 article-title: Maternal origin of erythrocytes in peripheral venous blood of pregnant women publication-title: Hum. Genet. – volume: 30 start-page: 1051 year: 1993 end-page: 1056 article-title: Prenatal diagnosis from maternal blood: simultaneous immunophenotyping and of fetal nucleated erythrocytes isolated by negative magnetic cell sorting publication-title: J. Med. Genet. – volume: 14 start-page: 291 year: 1999 end-page: 295 article-title: Improved specificity on NRBC detection in chorionic villus sample supernatant fluids using anti‐zeta and anti‐epsilon monoclonal antibodies publication-title: Fetal Diagn. Ther. – ident: e_1_2_5_3_2 doi: 10.1007/BF00214186 – ident: e_1_2_5_2_2 doi: 10.1136/jmg.30.12.1051 – ident: e_1_2_5_4_2 doi: 10.1159/000020942 |
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Snippet | : Fetal nucleated red blood cells (NRBCs) entering maternal circulation during pregnancy constitute a potential source of material for safe and reliable... Fetal nucleated red blood cells (NRBCs) entering maternal circulation during pregnancy constitute a potential source of material for safe and reliable... A bstract : Fetal nucleated red blood cells (NRBCs) entering maternal circulation during pregnancy constitute a potential source of material for safe and... |
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SubjectTerms | Anti-γ hemoglobin chain Ab Anti-ε hemoglobin chain Ab Antibodies, Monoclonal - immunology beta-Thalassemia - genetics Erythrocytes - immunology Female Fetus - metabolism Hemoglobins, Abnormal - immunology Heterozygote Humans Maternal-Fetal Exchange Noninvasive prenatal diagnosis NRBCs Pregnancy Pregnancy Trimester, First Pregnancy Trimester, Second Sensitivity and Specificity β-Thalassemia trait |
Title | Detection of Fetal NRBCs in Maternal Blood of Pregnant Carriers of β-Thalassemia Using Anti-γ and Anti-ε Monoclonal Antibodies |
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