Detection of Fetal NRBCs in Maternal Blood of Pregnant Carriers of β-Thalassemia Using Anti-γ and Anti-ε Monoclonal Antibodies

: Fetal nucleated red blood cells (NRBCs) entering maternal circulation during pregnancy constitute a potential source of material for safe and reliable noninvasive prenatal diagnosis. The increased prevalence of β‐thalassemia mutations in countries like Greece may create a problem, making it diffic...

Full description

Saved in:
Bibliographic Details
Published inAnnals of the New York Academy of Sciences Vol. 945; no. 1; pp. 151 - 152
Main Authors MAVROU, ARIADNI, KOLIALEXI, AGGELIKI, ANTSAKLIS, ARISTIDES, KRIKOS, XENOFON, KORATZIS, ASTERIS, METAXOTOU, CATHERINE
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.09.2001
Subjects
Online AccessGet full text

Cover

Loading…
Abstract : Fetal nucleated red blood cells (NRBCs) entering maternal circulation during pregnancy constitute a potential source of material for safe and reliable noninvasive prenatal diagnosis. The increased prevalence of β‐thalassemia mutations in countries like Greece may create a problem, making it difficult to distinguish between NRBCs of fetal or maternal origin. Use of Ab against embryonic hemoglobin ε may increase specificity for fetal NRBC detection. In the present study, Ab against embryonic hemoglobin ε was used in the first and second trimesters of pregnancy in order to determine if specificity for fetal NRBC detection could be increased.
AbstractList Fetal nucleated red blood cells (NRBCs) entering maternal circulation during pregnancy constitute a potential source of material for safe and reliable noninvasive prenatal diagnosis. The increased prevalence of beta-thalassemia mutations in countries like Greece may create a problem, making it difficult to distinguish between NRBCs of fetal or maternal origin. Use of Ab against embryonic hemoglobin epsilon may increase specificity for fetal NRBC detection. In the present study, Ab against embryonic hemoglobin epsilon was used in the first and second trimesters of pregnancy in order to determine if specificity for fetal NRBC detection could be increased.
: Fetal nucleated red blood cells (NRBCs) entering maternal circulation during pregnancy constitute a potential source of material for safe and reliable noninvasive prenatal diagnosis. The increased prevalence of β‐thalassemia mutations in countries like Greece may create a problem, making it difficult to distinguish between NRBCs of fetal or maternal origin. Use of Ab against embryonic hemoglobin ε may increase specificity for fetal NRBC detection. In the present study, Ab against embryonic hemoglobin ε was used in the first and second trimesters of pregnancy in order to determine if specificity for fetal NRBC detection could be increased.
A bstract : Fetal nucleated red blood cells (NRBCs) entering maternal circulation during pregnancy constitute a potential source of material for safe and reliable noninvasive prenatal diagnosis. The increased prevalence of β‐thalassemia mutations in countries like Greece may create a problem, making it difficult to distinguish between NRBCs of fetal or maternal origin. Use of Ab against embryonic hemoglobin ε may increase specificity for fetal NRBC detection. In the present study, Ab against embryonic hemoglobin ε was used in the first and second trimesters of pregnancy in order to determine if specificity for fetal NRBC detection could be increased.
Author MAVROU, ARIADNI
KORATZIS, ASTERIS
KOLIALEXI, AGGELIKI
METAXOTOU, CATHERINE
ANTSAKLIS, ARISTIDES
KRIKOS, XENOFON
Author_xml – sequence: 1
  givenname: ARIADNI
  surname: MAVROU
  fullname: MAVROU, ARIADNI
  email: ariamav@hol.gr
  organization: Medical Genetics, Athens University School of Medicine, Athens, Greece
– sequence: 2
  givenname: AGGELIKI
  surname: KOLIALEXI
  fullname: KOLIALEXI, AGGELIKI
  organization: Medical Genetics, Athens University School of Medicine, Athens, Greece
– sequence: 3
  givenname: ARISTIDES
  surname: ANTSAKLIS
  fullname: ANTSAKLIS, ARISTIDES
  organization: First Department of Obstetrics and Gynecology, Athens University School of Medicine, Athens, Greece
– sequence: 4
  givenname: XENOFON
  surname: KRIKOS
  fullname: KRIKOS, XENOFON
  organization: First Department of Pediatrics, Athens University School of Medicine, Athens, Greece
– sequence: 5
  givenname: ASTERIS
  surname: KORATZIS
  fullname: KORATZIS, ASTERIS
  organization: Department of Obstetrics and Gynecology, University of Patras School of Medicine, Patras, Greece
– sequence: 6
  givenname: CATHERINE
  surname: METAXOTOU
  fullname: METAXOTOU, CATHERINE
  organization: Medical Genetics, Athens University School of Medicine, Athens, Greece
BackLink https://www.ncbi.nlm.nih.gov/pubmed/11708470$$D View this record in MEDLINE/PubMed
BookMark eNqVkcFu1DAQhi1URLeFV0AWB24JdpzYCRe0XboFqbtdQQviZDnxuHjJ2sXOqtsjrwTiNfpMTbRROeOLZ8b_fJb-_wgdOO8AoVeUpLQ_b9YpFXmVcM6yNCOEpl1NWClEunuCJo9PB2hCiBBJWWXsEB3FuO6lWZmLZ-iQUkH6ikzQr_fQQdNZ77A3eA6davHy08ksYuvwQnUQXD85ab3Xg2AV4Nop1-GZCsFCiMPw_ndy-V21KkbYWIWvonXXeOo6m9z_wcrpsf6LF975pvUDcRjVXluIz9FTo9oIL8b7GF3NTy9nH5Lzi7OPs-l50uSkoklZlFQbXtU054bXhNfaMMNYQ7jRWvRtRnVVa24a0hRZwZgmBYCBGoiqK8WO0es99yb4n1uIndzY2EDbKgd-G6XIspLkjPXCt3thE3yMAYy8CXajwp2kRA4ByLUcXJaDy3IIQI4ByF2__HL8ZVtvQP9bHR3vBe_2glvbwt1_oOXy2_QzLWhPSPYEGzvYPRJU-CG5YKKQX5dncsXEalF8yeScPQCYCqpJ
CitedBy_id crossref_primary_10_1002_pd_1640
crossref_primary_10_1002_pd_1881
Cites_doi 10.1007/BF00214186
10.1136/jmg.30.12.1051
10.1159/000020942
ContentType Journal Article
DBID BSCLL
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7X8
DOI 10.1111/j.1749-6632.2001.tb03877.x
DatabaseName Istex
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
MEDLINE - Academic
DatabaseTitleList MEDLINE
MEDLINE - Academic

CrossRef
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Sciences (General)
Biology
EISSN 1749-6632
EndPage 152
ExternalDocumentID 10_1111_j_1749_6632_2001_tb03877_x
11708470
NYAS151
ark_67375_WNG_P37PM5V2_F
Genre article
Journal Article
GroupedDBID ---
--Z
-~X
.3N
.55
.GA
.GJ
.Y3
05W
0R~
10A
1CY
1OB
1OC
23M
31~
33P
3O-
3SF
4.4
50Y
50Z
51W
51X
52M
52N
52O
52P
52S
52T
52U
52W
52X
53G
5GY
5HH
5LA
5RE
5VS
66C
692
6J9
702
79B
7PT
8-0
8-1
8-3
8-4
8-5
8UM
930
A03
AAESR
AAEVG
AAHHS
AAMDK
AANLZ
AAONW
AASGY
AAXRX
AAZKR
ABCQN
ABCUV
ABDBF
ABEML
ABJNI
ABLJU
ABPVW
ACAHQ
ACBWZ
ACCFJ
ACCZN
ACGFO
ACGFS
ACIWK
ACPOU
ACPRK
ACSCC
ACXBN
ACXQS
ADBBV
ADEOM
ADIZJ
ADKYN
ADMGS
ADOJD
ADOZA
ADXAS
ADZMN
ADZOD
AEEZP
AEGXH
AEIGN
AEIMD
AELAQ
AENEX
AEQDE
AEUQT
AEUYR
AFBPY
AFFNX
AFFPM
AFGKR
AFPWT
AFRAH
AFSWV
AFZJQ
AHBTC
AHEFC
AHMBA
AI.
AIAGR
AITYG
AIURR
AIWBW
AJBDE
AJXKR
ALAGY
ALMA_UNASSIGNED_HOLDINGS
ALUQN
AMBMR
AMYDB
ATUGU
AUFTA
AZBYB
AZFZN
AZVAB
BAFTC
BDRZF
BFHJK
BHBCM
BIYOS
BMNLL
BMXJE
BNHUX
BROTX
BRXPI
BSCLL
BY8
C45
CAG
CO8
COF
CS3
D-E
D-F
DC6
DCZOG
DPXWK
DR2
DRFUL
DRSTM
EBD
EBS
EJD
EMOBN
ESX
F00
F01
F04
F5P
FEDTE
FZ0
G-S
G.N
GODZA
H.T
H.X
HF~
HGLYW
HVGLF
HZI
HZ~
I-F
IH2
IX1
J0M
K48
L7B
LATKE
LC2
LC3
LEEKS
LH4
LITHE
LOXES
LP6
LP7
LUTES
LW6
LYRES
MEWTI
MK4
MRFUL
MRSTM
MSFUL
MSSTM
MXFUL
MXSTM
N04
N05
N9A
NF~
NHB
O66
O9-
OHT
OIG
OK1
OVD
P2P
P2W
P2X
P4D
PALCI
PQQKQ
Q.N
Q11
QB0
R.K
RAG
RIWAO
RJQFR
ROL
RX1
S10
SAMSI
SJN
SUPJJ
SV3
TEORI
TUS
UB1
UPT
V8K
VH1
W8V
W99
WBKPD
WH7
WHWMO
WIH
WIK
WOHZO
WQJ
WRC
WUP
WVDHM
WXSBR
X7M
XG1
YBU
YOC
YSK
ZGI
ZKB
ZXP
ZZTAW
~02
~IA
~KM
~WT
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7X8
ID FETCH-LOGICAL-c4091-8581df69b146f6b06bdf3f33c06fdd76bd21d9bd6fc0c52533d05eefebe0ab9a3
IEDL.DBID DR2
ISSN 0077-8923
IngestDate Fri Aug 16 12:07:18 EDT 2024
Fri Aug 23 10:42:20 EDT 2024
Sat Sep 28 08:38:02 EDT 2024
Sat Aug 24 00:48:29 EDT 2024
Wed Oct 30 09:56:38 EDT 2024
IsPeerReviewed true
IsScholarly true
Issue 1
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c4091-8581df69b146f6b06bdf3f33c06fdd76bd21d9bd6fc0c52533d05eefebe0ab9a3
Notes istex:2F2A3D145B57B999EE9BAA49B0A42A3A1F298F2E
ark:/67375/WNG-P37PM5V2-F
ArticleID:NYAS151
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
PMID 11708470
PQID 72280433
PQPubID 23479
PageCount 2
ParticipantIDs proquest_miscellaneous_72280433
crossref_primary_10_1111_j_1749_6632_2001_tb03877_x
pubmed_primary_11708470
wiley_primary_10_1111_j_1749_6632_2001_tb03877_x_NYAS151
istex_primary_ark_67375_WNG_P37PM5V2_F
PublicationCentury 2000
PublicationDate September 2001
PublicationDateYYYYMMDD 2001-09-01
PublicationDate_xml – month: 09
  year: 2001
  text: September 2001
PublicationDecade 2000
PublicationPlace Oxford, UK
PublicationPlace_xml – name: Oxford, UK
– name: United States
PublicationTitle Annals of the New York Academy of Sciences
PublicationTitleAlternate Ann N Y Acad Sci
PublicationYear 2001
Publisher Blackwell Publishing Ltd
Publisher_xml – name: Blackwell Publishing Ltd
References Mavrou, A., A. Kolialexi et al. 1999. Improved specificity on NRBC detection in chorionic villus sample supernatant fluids using anti-zeta and anti-epsilon monoclonal antibodies. Fetal Diagn. Ther. 14: 291-295.
Slunga-Talberg, A., W. El-Rifai et al. 1995. Maternal origin of erythrocytes in peripheral venous blood of pregnant women. Hum. Genet. 96: 53-57.
Zheng, Y.L., N.P. Carter et al. 1993. Prenatal diagnosis from maternal blood: simultaneous immunophenotyping and FISH of fetal nucleated erythrocytes isolated by negative magnetic cell sorting. J. Med. Genet. 30: 1051-1056.
1995; 96
1993; 30
1999; 14
e_1_2_5_3_2
e_1_2_5_2_2
e_1_2_5_4_2
References_xml – volume: 96
  start-page: 53
  year: 1995
  end-page: 57
  article-title: Maternal origin of erythrocytes in peripheral venous blood of pregnant women
  publication-title: Hum. Genet.
– volume: 30
  start-page: 1051
  year: 1993
  end-page: 1056
  article-title: Prenatal diagnosis from maternal blood: simultaneous immunophenotyping and of fetal nucleated erythrocytes isolated by negative magnetic cell sorting
  publication-title: J. Med. Genet.
– volume: 14
  start-page: 291
  year: 1999
  end-page: 295
  article-title: Improved specificity on NRBC detection in chorionic villus sample supernatant fluids using anti‐zeta and anti‐epsilon monoclonal antibodies
  publication-title: Fetal Diagn. Ther.
– ident: e_1_2_5_3_2
  doi: 10.1007/BF00214186
– ident: e_1_2_5_2_2
  doi: 10.1136/jmg.30.12.1051
– ident: e_1_2_5_4_2
  doi: 10.1159/000020942
SSID ssj0012847
Score 1.7364912
Snippet : Fetal nucleated red blood cells (NRBCs) entering maternal circulation during pregnancy constitute a potential source of material for safe and reliable...
Fetal nucleated red blood cells (NRBCs) entering maternal circulation during pregnancy constitute a potential source of material for safe and reliable...
A bstract : Fetal nucleated red blood cells (NRBCs) entering maternal circulation during pregnancy constitute a potential source of material for safe and...
SourceID proquest
crossref
pubmed
wiley
istex
SourceType Aggregation Database
Index Database
Publisher
StartPage 151
SubjectTerms Anti-γ hemoglobin chain Ab
Anti-ε hemoglobin chain Ab
Antibodies, Monoclonal - immunology
beta-Thalassemia - genetics
Erythrocytes - immunology
Female
Fetus - metabolism
Hemoglobins, Abnormal - immunology
Heterozygote
Humans
Maternal-Fetal Exchange
Noninvasive prenatal diagnosis
NRBCs
Pregnancy
Pregnancy Trimester, First
Pregnancy Trimester, Second
Sensitivity and Specificity
β-Thalassemia trait
Title Detection of Fetal NRBCs in Maternal Blood of Pregnant Carriers of β-Thalassemia Using Anti-γ and Anti-ε Monoclonal Antibodies
URI https://api.istex.fr/ark:/67375/WNG-P37PM5V2-F/fulltext.pdf
https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1749-6632.2001.tb03877.x
https://www.ncbi.nlm.nih.gov/pubmed/11708470
https://search.proquest.com/docview/72280433
Volume 945
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3LbtQwFLVQERIboOXR8PQCIVhklIyTOFlOpwwV0oxGpYWysvyEUWmCJhmpsGLHlm8B8Rv9CL6Ee53MwKBuQCztxInt3Ht97BwfE_LQSZ4VzmShjhIbJqnSoczTNExgMLURhE1jcKI4nmR7h8nzo_So2x6Ne2FafYjVght6ho_X6OBS1etOzpMihBHT60nGuMbJcs57iChjxpHftbu_0pLycdiHZQ5hGWBNp0C6ovWc-6i10eoidvzpeVB0Hdn6oWl0lZwsG9UyUo57i0b19Mc_9B7_V6uvkSsdhqWD1ug2yQVbbpFL7amWH7bIZhcvavq4E7V-cp183rWNZ32VtHJ0ZAHz08n-zrCms5KOZStFTXeQRo83TOf2DVJ06FDO8Uy9GjPPvv749OXgLe78rO3JTFLPd6CDspnBhbNvVJZmlfpOIVxV-l3lqwqZqkLC5A1yOHp6MNwLu0MgQg1TzzjMU0DULisUhHSXqShTxjHHmI4yZwyHZD82hTKZ05FO-4BeTZRa68A4I6kKyW6SjbIq7TahUaIdWKNJVKwSLW1uCsSXjkvJ48TygLDlxxbvW60P8dscCfpdYL_jyZ2x6PpdnAbkkbeLVRE5P0a2HE_Fq8kzMWV8Ok5f9sUoIA-WhiPAh_HHjCxttagFR02ihLGA3Grt6dfrYx6B3UYByb1V_EW9xOT14AXgudv_XvQOudxS7pBSd5dsNPOFvQcYrFH3vW_9BLE7KIM
link.rule.ids 315,783,787,1378,27936,27937,46306,46730
linkProvider Wiley-Blackwell
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3LbtQwFLWqVgg2QMsrvOoFQrDIyJk8PFlOpwwDNKNRmUJZWXZst6NCguYhFVbs2PItIH6jH8GXcK-TCQzqBsQyb_vm3utj5-RcQh5YyZPU6sTPWWT8KFa5Lztx7EcwmBoGaVNrnChmw2RwED0_jA_XSLb8F6bSh2gW3DAyXL7GAMcF6dUo51Hqw5DpBCUDXOQMO5y3AFJuQPyHWMlhd79Rk3KZ2CVmDokZgE2tQdoQe86918p4tYGmPz0PjK5iWzc49a-QYtmtipNy0lrMVSv_-Ifi43_r91VyuYaxtFv53SZZM8UWuVAVtvywRTbrlDGjj2pd68fXyOddM3fEr4KWlvYNwH463N_pzeikoJms1KjpDjLp8YTR1BwhS4f25BTL6s1w59nXH5--jI_x58-ZeTeR1FEeaLeYT-DA2TcqC91sfaeQscr8bemaCjtViZzJ6-Sg_2TcG_h1HQg_h9ln4HdiANU2SRVkdZsolihtQxuGOUus1hw224FOlU5szvK4DQBWs9gYC_7JpEpleIOsF2VhbhHKotyCQ-pIBSrKpenoFCGm5VLyIDLcI-HybYv3ldyH-G2aBHYXaHcs3hmI2u7i1CMPnWM0l8jpCRLmeCxeD5-KUchHWfyqLfoe2V56joAwxm8zsjDlYiY4yhJFYeiRm5VD_Xp8wBk4LvNIx7nFX7RLDN90XwKku_3vl26Ti4Nxtif2ng1f3CGXKgYeMuzukvX5dGHuASSbq_su0H4CF3Ysmw
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3LbtQwFLVQKxAboOU1vOoFQrDIKJk4cbKcTgnlMaNRaaGsLDu221FpUk1mpMKKHVu-BcRv9CP4Eu51MoFB3YBY2okT27n3-tg5PibkoZU8Tq2OvdxnxmORyj2ZRJHHYDA1PoRNrXGiOBzF23vsxX6032yPxr0wtT5Eu-CGnuHiNTr4ibbLTs5Z6sGI6fQkA1zjDBPOu4AoV1kMUBgh0k4rJuUCsYvLHOIy4JpGgrTl9Zz7rKXhahV7_vQ8LLoMbd3YlF0lx4tW1ZSUo-58prr5xz8EH_9Xs6-RKw2Ipf3a6tbIBVOsk4v1sZYf1slaEzAq-rhRtX5ynXzeMjNH-ypoaWlmAPTT0c7moKKTgg5lrUVNN5FHjzeMp-YAOTp0IKd4qF6FmWdff3z6snuIWz8rczyR1BEeaL-YTeDC2TcqC92mvlOIV2X-vnRVhUxVImPyBtnLnu4Otr3mFAgvh7ln4CURQGobpwpiuo2VHyttQxuGuR9brTkke4FOlY5t7udRD-Cr9iNjLFinL1Uqw5tkpSgLc5tQn-UWzFEzFSiWS5PoFAGm5VLygBneIeHiY4uTWuxD_DZJgn4X2O94dGcgmn4Xpx3yyNlFW0ROj5AuxyPxdvRMjEM-HkZveiLrkI2F4QhwYvwzIwtTzivBUZSIhWGH3Krt6dfrA-6D3fodkjir-It6idG7_msAdHf-vegGuTTeysSr56OXd8nlmn6H9Lp7ZGU2nZv7gMdm6oFzs59uaCtK
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Detection+of+Fetal+NRBCs+in+Maternal+Blood+of+Pregnant+Carriers+of+%CE%B2-Thalassemia+Using+Anti-%CE%B3+and+Anti-%CE%B5+Monoclonal+Antibodies&rft.jtitle=Annals+of+the+New+York+Academy+of+Sciences&rft.au=MAVROU%2C+ARIADNI&rft.au=KOLIALEXI%2C+AGGELIKI&rft.au=ANTSAKLIS%2C+ARISTIDES&rft.au=KRIKOS%2C+XENOFON&rft.date=2001-09-01&rft.pub=Blackwell+Publishing+Ltd&rft.issn=0077-8923&rft.eissn=1749-6632&rft.volume=945&rft.issue=1&rft.spage=151&rft.epage=152&rft_id=info:doi/10.1111%2Fj.1749-6632.2001.tb03877.x&rft.externalDBID=n%2Fa&rft.externalDocID=ark_67375_WNG_P37PM5V2_F
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0077-8923&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0077-8923&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0077-8923&client=summon