Identification of the human salivary cystatin complex by the coupling of high-performance liquid chromatography and ion-trap mass spectrometry

• Published in: Proteomics (Weinheim) Vol. 3; no. 4; pp. 461 - 467
• Main Authors: Lupi, Alessandro, Messana, Irene, Denotti, Gloria, Schininà, Maria Eugenia, Gambarini, Gianluca, Fadda, Maria Benedetta, Vitali, Alberto, Cabras, Tiziana, Piras, Vincenzo, Patamia, Maria, Cordaro, Massimo, Giardina, Bruno, Castagnola, Massimo
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.04.2003; WILEY‐VCH Verlag; Wiley-VCH
• Subjects: Analysis of complex biological substances; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Blood and biological fluids; Chromatography, High Pressure Liquid - methods; Cystatins; Cystatins - analysis; Cystatins - chemistry; Fundamental and applied biological sciences. Psychology; Human saliva; Humans; Mass spectrometry; Saliva - chemistry; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization - methods; Human; Cystatin; Molecular form; HPLC chromatography; Identification; Saliva; Mass spectrometry

Human salivary cystatins, five major (S, S1, S2, SA, SN) and two minor (C and D), are multifunctional proteins playing a different role in the oral environment. Salivary cystatin SN is able to effectively inhibit lysosomal cathepsins B, C, H and L and cystatin SA inhibits cathepsins C and L in vitro...