Different N-Glycosylation Sites Reduce the Activity of Recombinant DSPAα2

• Published in: Current Issues in Molecular Biology Vol. 44; no. 9; pp. 3930 - 3947
• Main Authors: Peng, Huakang, Wang, Mengqi, Wang, Nan, Yang, Caifeng, Guo, Wenfang, Li, Gangqiang, Huang, Sumei, Wei, Di, Liu, Dehu
• Format: Journal Article
• Language: English
• Published: MDPI AG 31.08.2022; MDPI
• Subjects: DSPAα2; Fibrin; fibrin sensitivity; Immune response; N-glycosylation site; Pichia pastoris; plasminogen activator; China

Bat plasminogen activators α2 (DSPAα2) has extremely high medicinal value as a powerful natural thrombolytic protein. However, wild-type DSPAα2 has two N-glycosylation sites (N185 and N398) and its non-human classes of high-mannose-type N-glycans may cause immune responses in vivo. By mutating the N...