Time course evaluation of protein synthesis and glucose uptake after acute resistance exercise in rats
Noll Physiological Research Center and Graduate Program in Physiology, Pennsylvania State University, University Park, Pennsylvania 16802 The temporal pattern for changes in rates of protein synthesis and glucose uptake after resistance exercise, especially relative to each other, is not known. Male...
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Published in | Journal of applied physiology (1985) Vol. 88; no. 3; pp. 1142 - 1149 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
Am Physiological Soc
01.03.2000
American Physiological Society |
Subjects | |
Online Access | Get full text |
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Summary: | Noll Physiological Research Center and Graduate Program in
Physiology, Pennsylvania State University, University Park,
Pennsylvania 16802
The temporal pattern for changes in rates of protein
synthesis and glucose uptake after resistance exercise, especially
relative to each other, is not known. Male Sprague-Dawley rats
performed acute resistance exercise ( n = 7) or remained
sedentary ( n = 7 per group), and the following were assessed in
vivo 1, 3, 6, 12 and 24 h later: rates of protein synthesis, rates of
glucose uptake, phosphatidylinositol 3-kinase (PI3-kinase) activity,
and p70 S6k activity. Rates of protein synthesis in mixed
gastrocnemius muscle did not increase until 12 h after exercise (e.g.,
at 12 h, sedentary = 138 ± 4 vs. exercised = 178 ± 6 nmol
phenylalanine incorporated · g
muscle 1 · h 1 ,
mean ± SE, P < 0.05), whereas at 6 h after exercise rates
of glucose uptake were significantly elevated (sedentary = 0.18 ± 0.020 vs. exercised = 0.38 ± 0.024 µmol glucose 6-phosphate
incorporated · kg
muscle 1 · min 1 ,
P < 0.05). At 24 h after exercise, rates of protein synthesis were still elevated, whereas glucose uptake had returned to basal levels. Arterial insulin concentrations were not different between groups at any time. Non-insulin-stimulated activities of PI3-kinase and
p70 S6k were higher at 6, 12, and 24 h after exercise
( P < 0.05), and, generally, these occurred when rates of
protein synthesis (12 and 24 h) and glucose uptake were elevated (6 and
12 but not 24 h) by exercise. These data suggest that regulators of
protein synthesis and glucose uptake may respond to the same
contraction-generated signals with different kinetics or that they
respond to different intra- or extracellular signals that are generated
by exercise.
contractions; insulin |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 8750-7587 1522-1601 |
DOI: | 10.1152/jappl.2000.88.3.1142 |