A Fast Fluorescence Background Suppression Method for Raman Spectroscopy Based on Stepwise Spectral Reconstruction

• Published in: IEEE access Vol. 6; pp. 67709 - 67717
• Main Authors: Chen, Shuo, Kong, Lingmin, Xu, Wenbin, Cui, Xiaoyu, Liu, Quan
• Format: Journal Article
• Language: English
• Published: Piscataway IEEE 2018; The Institute of Electrical and Electronics Engineers, Inc. (IEEE)
• Subjects: Biological properties; Biomedical signal processing; Computational efficiency; Computing time; Fitting; Fluorescence; fluorescence suppression; Fourier transforms; Image reconstruction; Imaging; Nondestructive testing; Numerical analysis; Numerical methods; Polynomials; Raman imaging; Raman scattering; Raman spectra; Raman spectroscopy; Reconstruction; Spectrum analysis; Wavelet transforms

Raman spectroscopy is a rapid and non-destructive technique for detecting unique spectral fingerprints from biological samples. Raw Raman spectra often come with strong fluorescence background that makes spectral interpretation challenging. Although fluorescence background can be suppressed experime...