Primed CRISPR adaptation in Escherichia coli cells does not depend on conformational changes in the Cascade effector complex detected in Vitro

• Published in: Nucleic acids research Vol. 46; no. 8; pp. 4087 - 4098
• Main Authors: Krivoy, Andrey, Rutkauskas, Marius, Kuznedelov, Konstantin, Musharova, Olga, Rouillon, Christophe, Severinov, Konstantin, Seidel, Ralf
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 04.05.2018
• Subjects: Molecular Biology

Abstract In type I CRISPR-Cas systems, primed adaptation of new spacers into CRISPR arrays occurs when the effector Cascade-crRNA complex recognizes imperfectly matched targets that are not subject to efficient CRISPR interference. Thus, primed adaptation allows cells to acquire additional protectio...