Simultaneous nitrification and denitrification by Pseudomonas sp. Y-5 in a high nitrogen environment

• Published in: Environmental science and pollution research international Vol. 29; no. 46; pp. 69491 - 69501
• Main Authors: Zhang, Xiaoying, Xia, Yuxiang, Zeng, Yiwei, Sun, Xia, Tao, Ruidong, Mei, Yunjun, Qu, Mengjie
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.10.2022; Springer Nature B.V
• Subjects: Aerobic conditions; Ammonia; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Denitrification; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Municipal wastes; Municipal wastewater; Nitrates; Nitrification; Nitrites; Nitrogen; Nitrogen dioxide; Organic nitrogen; Pseudomonas; Research Article; Sewage disposal; sewage treatment; Sewage treatment plants; total nitrogen; Waste Water Technology; wastewater; Wastewater pollution; Wastewater treatment; Wastewater treatment plants; Water Management; Water Pollution Control; Simultaneous nitrification-denitrification; Nitrogen removal; sp. Y-5; Heterotrophic nitrification; Wastewater treatment; Aerobic denitrification; Pseudomonas sp. Y-5
• ISSN: 0944-1344; 1614-7499; 1614-7499
• DOI: 10.1007/s11356-022-20708-x

Pseudomonas sp. Y-5, a strain with simultaneous nitrification and denitrification (SND) capacity, was isolated from the Wuhan Municipal Sewage Treatment Plant. This strain could rapidly remove high concentrations of inorganic nitrogen. Specifically, Pseudomonas sp. Y-5 removed 103 mg/L of NH 4 + -N in 24 h without nitrate or nitrite accumulation when NH 4 + -N was its sole nitrogen source. The NH 4 + -N removal efficiency (RE) was 97.26%, and the average removal rate (RR) was 4.30 mg/L/h. Strain Y-5 also removed NO 3 − -N and NO 2 − -N even in aerobic conditions, with average RRs of 4.39 and 4.23 mg/L/h, respectively, and REs of up to 99.34% and 95.81% within 24 h. When cultured in SND medium (SNDM-1), strain Y-5 achieved an NH 4 + -N RE of up to 97.80% and a total nitrogen (TN) RE of 93.01%, whereas NO 3 − -N was fully depleted in 48 h. Interestingly, high nitrite concentrations did not inhibit the nitrification capacity of Y-5 when grown in SNDM-2, the RE of NH 4 + -N and TN reached 96.29% and 94.26%, respectively, and nitrite was consumed completely. Strain Y-5 also adapted well to high concentrations of ammonia (~401.68 mg NH 4 + -N/L) or organic nitrogen (~315.12 mg TN/L). Our results suggested that Pseudomonas sp. Y-5 achieved efficient simultaneous nitrification and denitrification, thus demonstrating its potential applicability in the treatment of nitrogen-polluted wastewater.