Aptamer redesigned tRNA is nonfunctional and degraded in cells

• Published in: RNA (Cambridge) Vol. 10; no. 1; pp. 7 - 11
• Main Authors: Lee, Dennis, McClain, William H
• Format: Journal Article
• Language: English
• Published: United States Copyright 2004 by RNA Society 01.01.2004
• Subjects: Acylation; Amino Acyl-tRNA Synthetases - genetics; Amino Acyl-tRNA Synthetases - metabolism; Cells, Cultured; Escherichia coli - enzymology; Escherichia coli - genetics; Glutamine - metabolism; Kinetics; Mutagenesis, Site-Directed; Mutation; Nucleic Acid Conformation; Peptide Chain Initiation, Translational - genetics; Plasmids; RNA aptamers; RNA, Bacterial - chemistry; RNA, Transfer, Gln - genetics; RNA, Transfer, Gln - metabolism

An RNA aptamer derived from tRNA(Gln) isolated in vitro and a rationally redesigned tRNA(Gln) were used to address the relationship between structure and function of tRNA(Gln) aminoacylation in Escherichia coli. Two mutant tRNA(Gln) sequences were studied: an aptamer that binds 26-fold tighter to gl...