Definition of a spliceosome interaction domain in yeast Prp2 ATPase

• Published in: RNA (Cambridge) Vol. 10; no. 2; pp. 210 - 220
• Main Authors: Edwalds-Gilbert, Gretchen, Kim, Dong-Ho, Silverman, Edward, Lin, Ren-Jang
• Format: Journal Article
• Language: English
• Published: United States Copyright 2004 by RNA Society 01.02.2004
• Subjects: Amino Acid Sequence; DEAD-box RNA Helicases; Galactose - metabolism; Molecular Sequence Data; Protein Structure, Tertiary; prp2 gene; RNA Splicing - physiology; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - metabolism; Spliceosomes - metabolism

The Saccharomyces cerevisiae splicing factor Prp2 is an RNA-dependent ATPase required before the first transesterification reaction in pre-mRNA splicing. Prp2 binds to the spliceosome in the absence of ATP and is released following ATP hydrolysis. It contains three domains: a unique N-terminal domai...