Interaction with the Nascent RNA Is a Prerequisite for the Recruitment of Rho to the Transcription Elongation Complex In Vitro

• Published in: Journal of molecular biology Vol. 413; no. 3; pp. 548 - 560
• Main Authors: Kalyani, B. Sudha, Muteeb, Ghazala, Qayyum, M. Zuhaib, Sen, Ranjan
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 28.10.2011
• Subjects: DNA-directed RNA polymerase; DNA-Directed RNA Polymerases - genetics; DNA-Directed RNA Polymerases - metabolism; Escherichia coli - genetics; Escherichia coli - metabolism; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; footprinting; mutants; Mutation - genetics; nucleotide sequences; Peptide Elongation Factors - genetics; Peptide Elongation Factors - metabolism; Rho; Rho Factor - genetics; Rho Factor - metabolism; ribonucleases; RNA; RNA polymerase; RNA, Bacterial - genetics; RNA, Bacterial - metabolism; rut site; Terminator Regions, Genetic; Transcription Factors - genetics; Transcription Factors - metabolism; transcription termination; Transcription, Genetic; Transcriptional Elongation Factors - metabolism; ATP-γS; AMPPNP; footprinting; transcription termination; RNA polymerase; RNAP; FT; RB; OC; Rho; rut site; WT; CHIP-chip; EC

In the conventional model of the Rho-dependent transcription termination, the terminator Rho binds to the rut (Rho utilization) site and translocates along the nascent RNA prior to making possible interactions with the elongating RNA polymerase (RNAP). Even though the interaction between Rho and iso...