Screening and Optimization of Zinc Removal Potential in Pseudomonas aeruginosa-HMR1 and its Plant Growth-Promoting Attributes

• Published in: Bulletin of environmental contamination and toxicology Vol. 108; no. 3; pp. 468 - 477
• Main Authors: Bhojiya, Ali Asger, Joshi, Harshada, Upadhyay, Sudhir K., Srivastava, Abhishek K., Pathak, Vinayak Vandan, Pandey, Vimal Chandra, Jain, Devendra
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.03.2022; Springer Nature B.V
• Subjects: Adsorption; Aquatic Pollution; Cadmium; Earth and Environmental Science; Ecotoxicology; electrical conductivity; Electrical resistivity; Environment; Environmental Chemistry; Environmental Health; Exopolysaccharides; Heavy metals; Iron; Lead; Metals, Heavy - analysis; Microscopic analysis; Nutrient concentrations; Optimization; Phylogeny; Plant growth; Pollution; Proline; Pseudomonas aeruginosa; Soil; Soil microorganisms; Soil Pollutants - analysis; Soil Science & Conservation; Solubilization; texture; toxicology; Waste Water Technology; Water Management; Water Pollution Control; Zinc; Zinc - analysis; Phylogenetic analysis; Zn-adsorption; Langmuir and Freundlich isotherms kinetics; Transmission-Electron-Microscope; Plant growth-promoting attributes; First and second-order kinetics; Pseudomonas aeruginosa
• ISSN: 0007-4861; 1432-0800; 1432-0800
• DOI: 10.1007/s00128-021-03232-5

The soil samples of old Zawar mine sites were sandy texture, basic, electric conductivity range from 16 to 59 dSm −1 with a high content of heavy metals of Zn, Pb, Cd, and Fe, indicating poor soil-health. Two bacterial isolates Pseudomonas aeruginosa HMR1 and P. aeruginosa HMR16 (GenBank-accession-number KJ191700 and KU174205, respectively), differed in the Phylogenetic tree based on 16S-rDNA sequences. HMR1 isolate showed the high potential of Plant growth-promoting attributes like IAA, Phosphate-solubilization, Exopolysaccharide production, and Proline activities at high concentration of Zn augmented nutrient media after 24 h, followed by HMR1 + HMR16 and HMR16. Both isolates were survived at 100 ppm Zn (w/v) concentration, followed by Pb, Cd, and Fe. Linear R L value from Langmuir and Freundlich isotherms revealed that the suitable condition of Zn adsorption by HMR1 was at pH8 with 40°C. The value of r 2 from pseudo-second-order kinetics and Transmission-Electron-Microscopic analysis confirmed Zn adsorption by HMR1.