Mucilaginibacter hurinus sp. nov., isolated from briquette warehouse soil

• Published in: Archives of microbiology Vol. 202; no. 1; pp. 127 - 134
• Main Authors: Choi, Lina, Zhao, Xinran, Song, Yali, Wu, Minghan, Wang, Gejiao, Li, Mingshun
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2020; Springer Nature B.V
• Subjects: agar; Bacterial Typing Techniques; Bacteroidetes - chemistry; Bacteroidetes - classification; Bacteroidetes - genetics; Base Composition; beta-glucosidase; Biochemistry; Biomedical and Life Sciences; Biotechnology; Briquets; Carbenicillin; Cell Biology; Cellobiase; chemotaxonomy; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Ecology; Fatty acids; Gene sequencing; genes; Genomes; Glucosamine; Glucosidase; Life Sciences; Lipids; Lipids - analysis; Menaquinones; Microbial Ecology; Microbiology; Mucilaginibacter; N-Acetylglucosamine; New species; nucleotide sequences; Original Paper; phenotype; Phosphatidylethanolamine; phosphatidylethanolamines; Phospholipids; Phylogenetics; Phylogeny; Piperacillin; Quinine; Republic of Korea; ribosomal RNA; RNA, Ribosomal, 16S - genetics; rRNA 16S; Salicin; Sequence Analysis, DNA; soil; Soil Microbiology; Soil microorganisms; Soils; South Korea; Species Specificity; sphingolipids; temperature; Warehouses; β-Glucosidase; Republic of Korea; South Korea; Polyphasic taxonomy; Mucilaginibacter hurinus
• DOI: 10.1007/s00203-019-01720-1
• ISSN: 0302-8933; 1432-072X

A novel bacterial strain, designated ZR32 T , was isolated from briquette warehouse soil in Ulsan (Korea). The strain was aerobic, showing pink-colored colonies on R2A agar. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ZR32 T was closely related to Mucilaginibacter soli R9-65 T (97.0%), Mucilaginibacter gynuensis YC7003 T (96.9%), and Mucilaginibacter lutimaris BR-3 T (96.8%). The values of DNA–DNA relatedness related two highest strains M. soli R9-65 T and M. gynuensis YC7003 T were 31.2 ± 6.9% and 19.7 ± 0.3%, respectively. Its genome size was 3.9 Mb, comprising 3402 predicted genes. The DNA G+C content of strain ZR32 T was 43.0 mol%. The major cellular fatty acids (> 5% of total) were summed feature 3 (C 16:1 ω 6 c and/or C 16:1 ω 7 c ), C 16:0 , C 16:1 ω 5 c , iso-C 15:0 , iso-C 17:0 3-OH, and C 17:1 ω 9 c . The major respiratory quinine was menaquinone-7 (MK-7). The major polar lipids were phosphatidylethanolamine, two unidentified phospholipids, one unidentified sphingolipid, and one unidentified polar lipid. Strain ZR32 T showed distinctive characteristics such as the temperature and pH for growth ranges, being positive for β-glucosidase, salicin production, negative for N -acetyl-glucosamine assimilation, being resistant to carbenicillin and piperacillin to related species. On the basis of phenotypic, chemotaxonomic, and phylogenetic data, strain ZR32 T represents a novel species of the genus Mucilaginibacter , for which the name Mucilaginibacter hurinus sp. nov. is proposed. The type strain is ZR32 T (= KCTC 62193 = CCTCC AB 2017285).