Downregulation of SERINC5 expression in buffy coats of HIV-1-infected patients with detectable or undetectable viral load

• Published in: Molecular biology reports Vol. 48; no. 5; pp. 4247 - 4252
• Main Authors: Hernández-López, Elsie Guadalupe, González-Enríquez, Gracia Viviana, Torres-Mendoza, Blanca Miriam, Cárdenas-Bedoya, Jhonathan, Escoto-Delgadillo, Martha, Vázquez-Valls, Eduardo, Pérez-Ríos, Alma Minerva, Carbajal-Uribe, David Alejandro, Rincón-Sánchez, Ana Rosa
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.05.2021; Springer Nature B.V
• Subjects: Adolescent; Adult; Aged; analysis of covariance; Animal Anatomy; Animal Biochemistry; antiretroviral agents; Antiretroviral drugs; Antiretroviral Therapy, Highly Active - methods; Biomedical and Life Sciences; Blood Buffy Coat - metabolism; Buffy coat; CD4 antigen; CD4-Positive T-Lymphocytes - metabolism; CD8 antigen; CD8-Positive T-Lymphocytes - metabolism; Cross-Sectional Studies; Down-Regulation - genetics; Female; Flow cytometry; gene expression; Highly active antiretroviral therapy; Histology; HIV; HIV infections; HIV Infections - blood; HIV Infections - drug therapy; HIV Infections - genetics; HIV Infections - virology; HIV-1 - genetics; Human immunodeficiency virus; Humans; Life Sciences; Lymphocytes T; Male; Membrane Proteins - blood; Membrane Proteins - genetics; Middle Aged; molecular biology; Morphology; mRNA; Original Article; Patients; quantitative polymerase chain reaction; Real-Time Polymerase Chain Reaction - methods; Regression analysis; Reverse Transcriptase Polymerase Chain Reaction - methods; T-lymphocytes; t-test; Treatment Outcome; viral load; Viral Load - methods; Young Adult; HIV-1; Naïve; SERINC5; Restriction factors; HAART
• ISSN: 0301-4851; 1573-4978; 1573-4978
• DOI: 10.1007/s11033-021-06438-2

Among the host restriction factors against HIV, SERINC5 has been described in vitro , but the mRNA level of SERINC5 in vivo has been little studied. We compare SERINC5 expression in subjects with HIV-1 (highly active antiretroviral treatment (HAART) and HAART-naïve) with and without suppression of viral load. A cross-sectional study was performed with 107 individuals distributed as follows: 24 with HAART-naïve and detectable viral load (> 50 copies/mL), 13 with HAART and detectable viral load (> 50 copies/mL), 50 with HAART and undetectable viral load (≤ 50 copies/mL), and 20 without HIV-1. SERINC5 expression in buffy coats was determined using RT-qPCR. The viral load was determined using real-time PCR and the amount of CD4 + and CD8 + T-lymphocytes was measured using flow cytometry. The data were normalized with the Shapiro–Wilk test and the Kruskal–Wallis test was subsequently performed. The relative expression was compared with a T-test and the remaining data with the Mann–Whitney U-test. ANCOVA multiple linear regression analysis was performed between characteristics of patients with SERINC5 expression. The mean and SD of the SERINC5 expression in the three groups with HIV-1 was 0.9 ± 0.2 and without HIV-1 was 1.7 ± 0.14 ( P  < 0.001). Multiple linear regression did not show the participation of CD4 +, CD8 + , viral load, infection time, or treatment time. No differences in the SERINC5 expression were found among the studied groups of patients with HIV-1. When comparing the groups with and without HIV-1 infection, SERINC5 was downregulation in the HIV-1 groups.