Molecular mechanisms of heavy metals resistance of Stenotrophomonas rhizophila JC1 by whole genome sequencing

• Published in: Archives of microbiology Vol. 203; no. 5; pp. 2699 - 2709
• Main Authors: Sun, Shang-Chen, Chen, Ji-Xiang, Wang, Yong-Gang, Leng, Fei-Fan, Zhao, Jian, Chen, Kai, Zhang, Qing-Chun
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.07.2021; Springer Nature B.V
• Subjects: ABC transporter; ABC transporters; Adenosine triphosphatase; adsorption; Arsenate reductase; Arsenates; Arsenic; bacteria; Biochemistry; Biomedical and Life Sciences; Biotechnology; carbohydrate binding; Carbohydrates; Cations; Cell Biology; China; Chromosomes; Copper; Detoxification; Divalent cations; Ecology; Efflux; Function analysis; Gene families; Gene sequencing; Genomes; Heavy metals; Homology; Lead; Life Sciences; metabolism; Metal ions; Metals; Microbial Ecology; Microbiology; Microorganisms; Molecular modelling; operon; Original Paper; oxidoreductases; polluted soils; Protein transport; Proteins; Reductases; Soil contamination; Soil pollution; Stenotrophomonas; Stenotrophomonas rhizophila; Thioredoxin; thioredoxins; Transcription; transcription factors; Whole genome sequencing; China; JC1; Adsorption; Genome; Heavy metals; Detoxification; Stenotrophomonas rhizophila JC1
• ISSN: 0302-8933; 1432-072X; 1432-072X
• DOI: 10.1007/s00203-021-02271-0

In this study, a higher metal ions-resistant bacterium, Stenotrophomonas rhizophila JC1 was isolated from contaminated soil in Jinchang city, Gansu Province, China. The Pb 2+ (120 mg/L) and Cu 2+ (80 mg/L) removal rate of the strain reached at 76.9% and 83.4%, respectively. The genome comprises 4268161 bp in a circular chromosome with 67.52% G + C content and encodes 3719 proteins. The genome function analysis showed czc operon, mer operon, cop operon, arsenic detoxification system in strain JC1 were contributed to the removal of heavy metals. Three efflux systems (i.e., RND, CDF, and P-ATPase) on strain JC1 genome could trigger the removal of divalent cations from cells. cAMP pathway and ABC transporter pathway might be involved in the transport and metabolism of heavy metals. The homology analysis exhibited multi-gene families such as ABC transporters, heavy metal-associated domain, copper resistance protein, carbohydrate-binding domain were distributed across 410 orthologous groups. In addition, heavy metal-responsive transcription regulator, thioredoxin, heavy metal transport/detoxification protein, divalent-cation resistance protein CutA, arsenate reductase also played important roles in the heavy metals adsorption and detoxification process. The complete genome data provides insight into the exploration of the interaction mechanism between microorganisms and heavy metals.