Human cell line that differentiates to all myeloid lineages and expresses neutrophil secondary granule genes

The aim of this study was to characterize a human leukemic cell line that appears capable of spontaneous differentiation to all myeloid lineages. The MPD cell line was derived using standard tissue culture techniques from the peripheral blood of a patient with an aggressive nonchronic myelogenous le...

Full description

Saved in:
Bibliographic Details
Published inExperimental hematology Vol. 28; no. 12; pp. 1373 - 1380
Main Authors Paul, Cassandra C, Aly, El-Sayed, Lehman, Jason A, Page, Scott M, Gomez-Cambronero, Julian, Ackerman, Steven J, Baumann, Michael A
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Inc 01.12.2000
Subjects
Online AccessGet full text

Cover

Loading…
Abstract The aim of this study was to characterize a human leukemic cell line that appears capable of spontaneous differentiation to all myeloid lineages. The MPD cell line was derived using standard tissue culture techniques from the peripheral blood of a patient with an aggressive nonchronic myelogenous leukemia myeloproliferative disorder. Immunophenotyping, cytogenetic analysis, reverse transcriptase polymerase chain reaction, Northern blotting, immunoblotting, and colony assays were used to characterize the line and to assess its ability to express lineage-specific genes representative of advanced differentiation. Light microscopic morphologic analysis of the MPD cell line suggests that it has the unique property of spontaneous differentiation to mature-appearing neutrophils, macrophages, eosinophils, and basophils in proportions that approximate those found in normal bone marrow or peripheral blood. It was demonstrated that this cell line is capable of producing lineage-specific mRNA and granule proteins of at least two myeloid lineages, neutrophil and eosinophil, including neutrophil secondary granule proteins, which are not expressed in other available human cell lines. MPD cells were found to be capable of producing differentiated myeloid colonies (neutrophil, eosinophil, macrophge, mixed) in semisolid medium. The ability of MPD cells to express genetic programs associated with advanced differentiation of multiple myeloid lineages will make it a valuable tool for the study of the processes underlying lineage commitment and the regulation of expression of lineage-specific genes.
AbstractList The aim of this study was to characterize a human leukemic cell line that appears capable of spontaneous differentiation to all myeloid lineages. The MPD cell line was derived using standard tissue culture techniques from the peripheral blood of a patient with an aggressive nonchronic myelogenous leukemia myeloproliferative disorder. Immunophenotyping, cytogenetic analysis, reverse transcriptase polymerase chain reaction, Northern blotting, immunoblotting, and colony assays were used to characterize the line and to assess its ability to express lineage-specific genes representative of advanced differentiation. Light microscopic morphologic analysis of the MPD cell line suggests that it has the unique property of spontaneous differentiation to mature-appearing neutrophils, macrophages, eosinophils, and basophils in proportions that approximate those found in normal bone marrow or peripheral blood. It was demonstrated that this cell line is capable of producing lineage-specific mRNA and granule proteins of at least two myeloid lineages, neutrophil and eosinophil, including neutrophil secondary granule proteins, which are not expressed in other available human cell lines. MPD cells were found to be capable of producing differentiated myeloid colonies (neutrophil, eosinophil, macrophge, mixed) in semisolid medium. The ability of MPD cells to express genetic programs associated with advanced differentiation of multiple myeloid lineages will make it a valuable tool for the study of the processes underlying lineage commitment and the regulation of expression of lineage-specific genes.
The aim of this study was to characterize a human leukemic cell line that appears capable of spontaneous differentiation to all myeloid lineages. The MPD cell line was derived using standard tissue culture techniques from the peripheral blood of a patient with an aggressive nonchronic myelogenous leukemia myeloproliferative disorder. Immunophenotyping, cytogenetic analysis, reverse transcriptase polymerase chain reaction, Northern blotting, immunoblotting, and colony assays were used to characterize the line and to assess its ability to express lineage-specific genes representative of advanced differentiation.Light microscopic morphologic analysis of the MPD cell line suggests that it has the unique property of spontaneous differentiation to mature-appearing neutrophils, macrophages, eosinophils, and basophils in proportions that approximate those found in normal bone marrow or peripheral blood. It was demonstrated that this cell line is capable of producing lineage-specific mRNA and granule proteins of at least two myeloid lineages, neutrophil and eosinophil, including neutrophil secondary granule proteins, which are not expressed in other available human cell lines. MPD cells were found to be capable of producing differentiated myeloid colonies (neutrophil, eosinophil, macrophge, mixed) in semisolid medium. The ability of MPD cells to express genetic programs associated with advanced differentiation of multiple myeloid lineages will make it a valuable tool for the study of the processes underlying lineage commitment and the regulation of expression of lineage-specific genes.
Author Lehman, Jason A
Aly, El-Sayed
Ackerman, Steven J
Page, Scott M
Paul, Cassandra C
Baumann, Michael A
Gomez-Cambronero, Julian
Author_xml – sequence: 1
  givenname: Cassandra C
  surname: Paul
  fullname: Paul, Cassandra C
  organization: Research Service, Department of Veterans Affairs, the Departments of Medicine and, Wright State University, Dayton, OH, USA
– sequence: 2
  givenname: El-Sayed
  surname: Aly
  fullname: Aly, El-Sayed
  organization: Research Service, Department of Veterans Affairs, the Departments of Medicine and, Wright State University, Dayton, OH, USA
– sequence: 3
  givenname: Jason A
  surname: Lehman
  fullname: Lehman, Jason A
  organization: Research Service, Department of Veterans Affairs, the Departments of Physiology and Biophysics, Wright State University, Dayton, OH, USA
– sequence: 4
  givenname: Scott M
  surname: Page
  fullname: Page, Scott M
  organization: Departments of Biochemistry and Molecular Biology and Medicine, The University of Illinois at Chicago, Chicago, Ill., USA
– sequence: 5
  givenname: Julian
  surname: Gomez-Cambronero
  fullname: Gomez-Cambronero, Julian
  organization: Research Service, Department of Veterans Affairs, the Departments of Physiology and Biophysics, Wright State University, Dayton, OH, USA
– sequence: 6
  givenname: Steven J
  surname: Ackerman
  fullname: Ackerman, Steven J
  organization: Departments of Biochemistry and Molecular Biology and Medicine, The University of Illinois at Chicago, Chicago, Ill., USA
– sequence: 7
  givenname: Michael A
  surname: Baumann
  fullname: Baumann, Michael A
  email: baumann.michael@dayton.va.gov
  organization: Research Service, Department of Veterans Affairs, the Departments of Medicine and, Wright State University, Dayton, OH, USA
BackLink https://www.ncbi.nlm.nih.gov/pubmed/11146159$$D View this record in MEDLINE/PubMed
BookMark eNqFkM9vFSEQgImpsa_VP0HDyehhKwO7C5yMabRt0qSHavJuhMLsK2YXnsA29r9334_o0dNkhm9mmO-MnMQUkZC3wC6AQf_pngkGTSv5-gNjHxnrOt6sX5AVKCkaLrQ-Iau_yCk5K-Un21GavSKnAND20OkVGa_nyUbqcBzpGCLS-mgr9WEYMGOswVYstCZql_fpGccU_J6zm6Vuo6f4e5uxlCWLONecto9hpAVdit7mZ7rJNs4j0g1GLK_Jy8GOBd8c4zn58e3r98vr5vbu6ubyy23jWqZq03s1SI2IutPSKxAOGO-5FQ-t6h23HgYN0nkhpRKd5NopNXRuEMz1PXAQ5-T9Ye42p18zlmqmUHYn2ohpLkbytlcC9AJ2B9DlVErGwWxzmJZ_G2Bmp9nsNZudQ8OY2Ws266Xv3XHB_DCh_9d19LoAnw8ALmc-BcymuIDRoQ8ZXTU-hf-s-AOX9ZBT
CitedBy_id crossref_primary_10_1189_jlb_0109042
crossref_primary_10_1186_1471_2407_13_364
crossref_primary_10_4049_jimmunol_171_12_6846
crossref_primary_10_1189_jlb_72_3_478
crossref_primary_10_1016_j_gene_2005_04_032
crossref_primary_10_1080_1042819032000159924
crossref_primary_10_1016_j_leukres_2004_05_009
crossref_primary_10_1002_gcc_10144
crossref_primary_10_1002_ajh_1160
crossref_primary_10_1016_j_isci_2020_101724
crossref_primary_10_1016_j_leukres_2003_11_012
Cites_doi 10.1182/blood.V84.1.294.294
10.1182/blood.V91.7.2517
10.1016/0145-2126(88)90103-8
10.4049/jimmunol.160.3.1385
10.1084/jem.183.5.2349
10.1074/jbc.271.22.13088
10.1182/blood.V85.3.812.bloodjournal853812
10.1182/blood.V70.5.1233.1233
10.1002/jlb.37.4.407
10.1182/blood.V81.11.2844.2844
10.1182/blood.V79.11.2998.2998
10.1182/blood.V80.3.788.788
10.1002/jlb.56.1.74
10.1002/stem.160016
10.1111/j.1365-2141.1994.tb04904.x
10.1182/blood.V91.12.4543.412k27_4543_4553
ContentType Journal Article
Copyright 2000 International Society for Experimental Hematology.
Copyright_xml – notice: 2000 International Society for Experimental Hematology.
DBID CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7X8
DOI 10.1016/S0301-472X(00)00552-X
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
MEDLINE - Academic
DatabaseTitleList
MEDLINE
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Medicine
EISSN 1873-2399
EndPage 1380
ExternalDocumentID 10_1016_S0301_472X_00_00552_X
11146159
S0301472X0000552X
Genre Research Support, U.S. Gov't, Non-P.H.S
Research Support, U.S. Gov't, P.H.S
Research Support, Non-U.S. Gov't
Journal Article
Case Reports
GrantInformation_xml – fundername: NIAID NIH HHS
  grantid: AI33043
– fundername: NHLBI NIH HHS
  grantid: HL056653
– fundername: NIAID NIH HHS
  grantid: AI25230
GroupedDBID ---
--K
--M
.1-
.55
.FO
.GJ
.~1
0R~
1B1
1P~
1RT
1~.
1~5
29G
4.4
457
4G.
53G
5GY
5RE
5VS
7-5
71M
8P~
AABNK
AACTN
AAEDT
AAEDW
AAIAV
AAIKJ
AAKOC
AALRI
AAOAW
AAQFI
AAQXK
AAXUO
ABBQC
ABCQX
ABFNM
ABGSF
ABJNI
ABLVK
ABMAC
ABMZM
ABUDA
ABXDB
ABYKQ
ACDAQ
ACIUM
ACRLP
ADBBV
ADEZE
ADMUD
ADUVX
AEBSH
AEHWI
AEKER
AENEX
AEVXI
AFCTW
AFFNX
AFKWA
AFRHN
AFTJW
AFXIZ
AGHFR
AGRDE
AGUBO
AGYEJ
AHPSJ
AIEXJ
AIKHN
AITUG
AJBFU
AJOXV
AJRQY
AJUYK
ALMA_UNASSIGNED_HOLDINGS
AMFUW
AMRAJ
ANZVX
ASPBG
AVWKF
AXJTR
AZFZN
BKOJK
BLXMC
BNPGV
CS3
DOVZS
EBS
EFJIC
EFLBG
EJD
EO8
EO9
EP2
EP3
F5P
FDB
FEDTE
FGOYB
FIRID
FNPLU
FYGXN
G-Q
GBLVA
HVGLF
HX~
HZ~
IHE
J1W
K-O
KOM
L7B
LCYCR
M41
MO0
N9A
O-L
O9-
OAUVE
OC~
OO-
OZT
P-8
P-9
P2P
PC.
Q38
R2-
RIG
ROL
RPZ
SCC
SDF
SDG
SDH
SDP
SEL
SES
SEW
SPCBC
SSH
SSU
SSZ
T5K
UDS
X7M
Y6R
Z5R
ZGI
ZXP
~G-
0SF
AAXKI
ADVLN
AFJKZ
AKRWK
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
ACRPL
ADNMO
CITATION
7X8
ID FETCH-LOGICAL-c408t-6d8f79eee9597d813c10262a3b486c2ad1f917cd377835729c88f5cf30c661213
IEDL.DBID .~1
ISSN 0301-472X
IngestDate Fri Oct 25 11:57:14 EDT 2024
Fri Dec 06 04:08:05 EST 2024
Sat Sep 28 07:35:34 EDT 2024
Fri Feb 23 02:29:42 EST 2024
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 12
Keywords Cell line
Neutrophil
Secondary granules
Differentiation
eosinophil
Language English
License http://www.elsevier.com/open-access/userlicense/1.0
https://www.elsevier.com/tdm/userlicense/1.0
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c408t-6d8f79eee9597d813c10262a3b486c2ad1f917cd377835729c88f5cf30c661213
Notes ObjectType-Case Study-3
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-4
content type line 23
ObjectType-Report-2
OpenAccessLink http://www.exphem.org/article/S0301472X0000552X/pdf
PMID 11146159
PQID 72468319
PQPubID 23479
PageCount 8
ParticipantIDs proquest_miscellaneous_72468319
crossref_primary_10_1016_S0301_472X_00_00552_X
pubmed_primary_11146159
elsevier_sciencedirect_doi_10_1016_S0301_472X_00_00552_X
PublicationCentury 2000
PublicationDate 2000-12-01
PublicationDateYYYYMMDD 2000-12-01
PublicationDate_xml – month: 12
  year: 2000
  text: 2000-12-01
  day: 01
PublicationDecade 2000
PublicationPlace Netherlands
PublicationPlace_xml – name: Netherlands
PublicationTitle Experimental hematology
PublicationTitleAlternate Exp Hematol
PublicationYear 2000
Publisher Elsevier Inc
Publisher_xml – name: Elsevier Inc
References Ogawa (BIB1) 1993; 81
Lawson, Khanna-Gupta, Berliner (BIB2) 1998; 91
Joseph, Paul, Baumann, Gomez-Cambronero (BIB7) 1996; 271
Tiffany, Alkhatib, Combadiere, Berger, Murphy (BIB11) 1998; 160
Paul, Ackerman, Mahrer, Tolbert, Dvorak, Baumann (BIB9) 1994; 56
Fischkoff (BIB14) 1998; 12
Collins (BIB16) 1987; 70
Lawson, Krause, Berliner (BIB5) 1998; 26
Khanna-Gupta, Zibello, Berliner (BIB13) 1996; 211
Khanna-Gupta, Kolibaba, Zibello, Berliner (BIB3) 1994; 84
Borregaard, Sehested, Nielsen, Sengeløv, Kjeldsen (BIB8) 1995; 85
Baumann, Paul (BIB10) 1998; 16
Johnston, Rintels, Chung, Sather, Benz, Berliner (BIB4) 1992; 79
Harris, Ralph (BIB17) 1985; 37
Fabian, Lass, Kletter, Golde (BIB15) 1992; 80
Tohyama, Tsutani, Ueda, Nakamura, Yoshida (BIB18) 1994; 87
Lawson, Khanna-Gupta, Berliner (BIB12) 1998; 91
Daugherty, Siciliano, Demartino, Malkowitz, Sirotina, Springer (BIB6) 1996; 183
Ikeda, Sasaki, Ideda (BIB19) 1998; 91
Borregaard (10.1016/S0301-472X(00)00552-X_BIB8) 1995; 85
Tohyama (10.1016/S0301-472X(00)00552-X_BIB18) 1994; 87
Khanna-Gupta (10.1016/S0301-472X(00)00552-X_BIB13) 1996; 211
Daugherty (10.1016/S0301-472X(00)00552-X_BIB6) 1996; 183
Collins (10.1016/S0301-472X(00)00552-X_BIB16) 1987; 70
Lawson (10.1016/S0301-472X(00)00552-X_BIB12) 1998; 91
Khanna-Gupta (10.1016/S0301-472X(00)00552-X_BIB3) 1994; 84
Harris (10.1016/S0301-472X(00)00552-X_BIB17) 1985; 37
Fabian (10.1016/S0301-472X(00)00552-X_BIB15) 1992; 80
Paul (10.1016/S0301-472X(00)00552-X_BIB9) 1994; 56
Lawson (10.1016/S0301-472X(00)00552-X_BIB5) 1998; 26
Ogawa (10.1016/S0301-472X(00)00552-X_BIB1) 1993; 81
Tiffany (10.1016/S0301-472X(00)00552-X_BIB11) 1998; 160
Ikeda (10.1016/S0301-472X(00)00552-X_BIB19) 1998; 91
Joseph (10.1016/S0301-472X(00)00552-X_BIB7) 1996; 271
Fischkoff (10.1016/S0301-472X(00)00552-X_BIB14) 1998; 12
Lawson (10.1016/S0301-472X(00)00552-X_BIB2) 1998; 91
Johnston (10.1016/S0301-472X(00)00552-X_BIB4) 1992; 79
Baumann (10.1016/S0301-472X(00)00552-X_BIB10) 1998; 16
References_xml – volume: 16
  start-page: 16
  year: 1998
  ident: BIB10
  article-title: The aml14 and aml14.3D10 cell lines
  publication-title: Stem Cells
  contributor:
    fullname: Paul
– volume: 91
  start-page: 4543
  year: 1998
  ident: BIB19
  article-title: A new cytokine-dependent monoblastic cell line with (9;11)(p22;q23) differentiates to macrophages with macrophage colony-stimulating factor (M-CSF) and to osteoclast-like cells with M-CSF and interleukin-4
  publication-title: Blood
  contributor:
    fullname: Ideda
– volume: 70
  start-page: 1233
  year: 1987
  ident: BIB16
  article-title: The HL-60 promyelocytic leukemia cell line
  publication-title: Blood
  contributor:
    fullname: Collins
– volume: 183
  start-page: 2349
  year: 1996
  ident: BIB6
  article-title: Cloning, expression and characterization of the human eosinophil eotaxin receptor
  publication-title: J Exp Med
  contributor:
    fullname: Springer
– volume: 91
  start-page: 2517
  year: 1998
  ident: BIB12
  article-title: Isolation and characterization of the cDNA for mouse neutrophil collagenase
  publication-title: Blood
  contributor:
    fullname: Berliner
– volume: 91
  start-page: 2517
  year: 1998
  ident: BIB2
  article-title: Isolation and characterization of the cDNA for mouse neutrophil collagenase
  publication-title: Blood
  contributor:
    fullname: Berliner
– volume: 56
  start-page: 74
  year: 1994
  ident: BIB9
  article-title: Cytokine induction of granule protein synthesis in an eosinophil-inducible human myeloid cell line, AML14
  publication-title: J Leukoc Biol
  contributor:
    fullname: Baumann
– volume: 87
  start-page: 235
  year: 1994
  ident: BIB18
  article-title: Establishment and characterization of a novel myeloid cell line from the bone marrow of a patient with myelodysplastic syndrome
  publication-title: Br J Hematol
  contributor:
    fullname: Yoshida
– volume: 211
  start-page: 165
  year: 1996
  ident: BIB13
  article-title: Coordinate regulation of neutrophil secondary granule protein gene expression
  publication-title: Curr Top Microbiol Immunol
  contributor:
    fullname: Berliner
– volume: 271
  start-page: 13088
  year: 1996
  ident: BIB7
  article-title: S6 kinase p90
  publication-title: J Biol Chem
  contributor:
    fullname: Gomez-Cambronero
– volume: 12
  start-page: 679
  year: 1998
  ident: BIB14
  article-title: Graded increase in probability of eosinophilic differentiation of HL-60 promyelocytic leukemia cells induced by culture under alkaline conditions
  publication-title: Leuk Res
  contributor:
    fullname: Fischkoff
– volume: 80
  start-page: 788
  year: 1992
  ident: BIB15
  article-title: Differentiation and functional activity of human eosinophilic cells from an eosinophil HL-60 subline
  publication-title: Blood
  contributor:
    fullname: Golde
– volume: 85
  start-page: 812
  year: 1995
  ident: BIB8
  article-title: Biosynthesis of granule proteins in normal human bone marrow cells. Gelatinase is a marker of terminal neutrophil differentiation
  publication-title: Blood
  contributor:
    fullname: Kjeldsen
– volume: 84
  start-page: 294
  year: 1994
  ident: BIB3
  article-title: NB4 cells show bilineage potential and an aberrant pattern of neutrophil secondary granule protein gene expression
  publication-title: Blood
  contributor:
    fullname: Berliner
– volume: 79
  start-page: 2998
  year: 1992
  ident: BIB4
  article-title: Lactoferrin gene promoter
  publication-title: Blood
  contributor:
    fullname: Berliner
– volume: 26
  start-page: 1178
  year: 1998
  ident: BIB5
  article-title: Normal neutrophil differentiation and secondary granule gene expression in the EML and MPRO cell lines
  publication-title: Exp Hematol
  contributor:
    fullname: Berliner
– volume: 37
  start-page: 407
  year: 1985
  ident: BIB17
  article-title: Human leukemic models of myelomonocytic development
  publication-title: J Leukoc Biol
  contributor:
    fullname: Ralph
– volume: 81
  start-page: 2844
  year: 1993
  ident: BIB1
  article-title: Differentiation and proliferation of hematopoietic stem cells
  publication-title: Blood
  contributor:
    fullname: Ogawa
– volume: 160
  start-page: 1385
  year: 1998
  ident: BIB11
  article-title: CC chemokine receptors 1 and 3 are differentially regulated by IL-5 during maturation of eosinophilic HL-60 cells
  publication-title: J Immunol
  contributor:
    fullname: Murphy
– volume: 84
  start-page: 294
  year: 1994
  ident: 10.1016/S0301-472X(00)00552-X_BIB3
  article-title: NB4 cells show bilineage potential and an aberrant pattern of neutrophil secondary granule protein gene expression
  publication-title: Blood
  doi: 10.1182/blood.V84.1.294.294
  contributor:
    fullname: Khanna-Gupta
– volume: 91
  start-page: 2517
  year: 1998
  ident: 10.1016/S0301-472X(00)00552-X_BIB12
  article-title: Isolation and characterization of the cDNA for mouse neutrophil collagenase
  publication-title: Blood
  doi: 10.1182/blood.V91.7.2517
  contributor:
    fullname: Lawson
– volume: 12
  start-page: 679
  year: 1998
  ident: 10.1016/S0301-472X(00)00552-X_BIB14
  article-title: Graded increase in probability of eosinophilic differentiation of HL-60 promyelocytic leukemia cells induced by culture under alkaline conditions
  publication-title: Leuk Res
  doi: 10.1016/0145-2126(88)90103-8
  contributor:
    fullname: Fischkoff
– volume: 160
  start-page: 1385
  year: 1998
  ident: 10.1016/S0301-472X(00)00552-X_BIB11
  article-title: CC chemokine receptors 1 and 3 are differentially regulated by IL-5 during maturation of eosinophilic HL-60 cells
  publication-title: J Immunol
  doi: 10.4049/jimmunol.160.3.1385
  contributor:
    fullname: Tiffany
– volume: 26
  start-page: 1178
  year: 1998
  ident: 10.1016/S0301-472X(00)00552-X_BIB5
  article-title: Normal neutrophil differentiation and secondary granule gene expression in the EML and MPRO cell lines
  publication-title: Exp Hematol
  contributor:
    fullname: Lawson
– volume: 183
  start-page: 2349
  year: 1996
  ident: 10.1016/S0301-472X(00)00552-X_BIB6
  article-title: Cloning, expression and characterization of the human eosinophil eotaxin receptor
  publication-title: J Exp Med
  doi: 10.1084/jem.183.5.2349
  contributor:
    fullname: Daugherty
– volume: 271
  start-page: 13088
  year: 1996
  ident: 10.1016/S0301-472X(00)00552-X_BIB7
  article-title: S6 kinase p90rsk in granulocyte-macrophage colony-stimulating factor-stimulated proliferative and mature hematopoietic cells
  publication-title: J Biol Chem
  doi: 10.1074/jbc.271.22.13088
  contributor:
    fullname: Joseph
– volume: 85
  start-page: 812
  year: 1995
  ident: 10.1016/S0301-472X(00)00552-X_BIB8
  article-title: Biosynthesis of granule proteins in normal human bone marrow cells. Gelatinase is a marker of terminal neutrophil differentiation
  publication-title: Blood
  doi: 10.1182/blood.V85.3.812.bloodjournal853812
  contributor:
    fullname: Borregaard
– volume: 211
  start-page: 165
  year: 1996
  ident: 10.1016/S0301-472X(00)00552-X_BIB13
  article-title: Coordinate regulation of neutrophil secondary granule protein gene expression
  publication-title: Curr Top Microbiol Immunol
  contributor:
    fullname: Khanna-Gupta
– volume: 91
  start-page: 2517
  year: 1998
  ident: 10.1016/S0301-472X(00)00552-X_BIB2
  article-title: Isolation and characterization of the cDNA for mouse neutrophil collagenase
  publication-title: Blood
  doi: 10.1182/blood.V91.7.2517
  contributor:
    fullname: Lawson
– volume: 70
  start-page: 1233
  year: 1987
  ident: 10.1016/S0301-472X(00)00552-X_BIB16
  article-title: The HL-60 promyelocytic leukemia cell line
  publication-title: Blood
  doi: 10.1182/blood.V70.5.1233.1233
  contributor:
    fullname: Collins
– volume: 37
  start-page: 407
  year: 1985
  ident: 10.1016/S0301-472X(00)00552-X_BIB17
  article-title: Human leukemic models of myelomonocytic development
  publication-title: J Leukoc Biol
  doi: 10.1002/jlb.37.4.407
  contributor:
    fullname: Harris
– volume: 81
  start-page: 2844
  year: 1993
  ident: 10.1016/S0301-472X(00)00552-X_BIB1
  article-title: Differentiation and proliferation of hematopoietic stem cells
  publication-title: Blood
  doi: 10.1182/blood.V81.11.2844.2844
  contributor:
    fullname: Ogawa
– volume: 79
  start-page: 2998
  year: 1992
  ident: 10.1016/S0301-472X(00)00552-X_BIB4
  article-title: Lactoferrin gene promoter
  publication-title: Blood
  doi: 10.1182/blood.V79.11.2998.2998
  contributor:
    fullname: Johnston
– volume: 80
  start-page: 788
  year: 1992
  ident: 10.1016/S0301-472X(00)00552-X_BIB15
  article-title: Differentiation and functional activity of human eosinophilic cells from an eosinophil HL-60 subline
  publication-title: Blood
  doi: 10.1182/blood.V80.3.788.788
  contributor:
    fullname: Fabian
– volume: 56
  start-page: 74
  year: 1994
  ident: 10.1016/S0301-472X(00)00552-X_BIB9
  article-title: Cytokine induction of granule protein synthesis in an eosinophil-inducible human myeloid cell line, AML14
  publication-title: J Leukoc Biol
  doi: 10.1002/jlb.56.1.74
  contributor:
    fullname: Paul
– volume: 16
  start-page: 16
  year: 1998
  ident: 10.1016/S0301-472X(00)00552-X_BIB10
  article-title: The aml14 and aml14.3D10 cell lines
  publication-title: Stem Cells
  doi: 10.1002/stem.160016
  contributor:
    fullname: Baumann
– volume: 87
  start-page: 235
  year: 1994
  ident: 10.1016/S0301-472X(00)00552-X_BIB18
  article-title: Establishment and characterization of a novel myeloid cell line from the bone marrow of a patient with myelodysplastic syndrome
  publication-title: Br J Hematol
  doi: 10.1111/j.1365-2141.1994.tb04904.x
  contributor:
    fullname: Tohyama
– volume: 91
  start-page: 4543
  year: 1998
  ident: 10.1016/S0301-472X(00)00552-X_BIB19
  article-title: A new cytokine-dependent monoblastic cell line with (9;11)(p22;q23) differentiates to macrophages with macrophage colony-stimulating factor (M-CSF) and to osteoclast-like cells with M-CSF and interleukin-4
  publication-title: Blood
  doi: 10.1182/blood.V91.12.4543.412k27_4543_4553
  contributor:
    fullname: Ikeda
SSID ssj0005590
Score 1.7146393
Snippet The aim of this study was to characterize a human leukemic cell line that appears capable of spontaneous differentiation to all myeloid lineages. The MPD cell...
The aim of this study was to characterize a human leukemic cell line that appears capable of spontaneous differentiation to all myeloid lineages. The MPD cell...
SourceID proquest
crossref
pubmed
elsevier
SourceType Aggregation Database
Index Database
Publisher
StartPage 1373
SubjectTerms Basophils - pathology
Cell Differentiation
Cell line
Cytoplasmic Granules - genetics
Differentiation
eosinophil
Eosinophils - pathology
Gene Expression
Granulocyte Colony-Stimulating Factor - pharmacology
Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology
Granulocytes - pathology
Humans
Leukemia, Myeloid, Acute - pathology
Leukocyte Count
Macrophages - pathology
Male
Middle Aged
Myeloproliferative Disorders - pathology
Neutrophil
Neutrophils - ultrastructure
RNA, Messenger - biosynthesis
Secondary granules
Tumor Cells, Cultured
Title Human cell line that differentiates to all myeloid lineages and expresses neutrophil secondary granule genes
URI https://dx.doi.org/10.1016/S0301-472X(00)00552-X
https://www.ncbi.nlm.nih.gov/pubmed/11146159
https://search.proquest.com/docview/72468319
Volume 28
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LS8QwEA6iIF7Et-szBw96qJtt0zZ7lEVZn4gP6C2kSaoLa7vsdkEv_nZn0pbVgwieStO0DZPkyzeTmQwhRzwEkp9x5nVFBxQUFWhPxcZ6meUWt_1E6pLB3N5F_Wd-lYTJHOk1sTDoVlljf4XpDq3rknYtzfZoMGg_Om0g9hOE3DD0E4xgDxlmMTj9_ObmEVZ2FqjsYe1ZFE_1BVd4zNiJ-4iX_LY-_cY_3Tp0sUKWawJJz6o2rpI5m6-Rxdt6i3ydDJ1ZnqJBniKFpOWrKmmTBwXmM3BLWhZUwfO3DzssBsbVA1yZUJUbat-dbyzc5XZajovR62BIJ6g3GzX-oC-wuE2Hlr4gSG6Q54vzp17fq1MqeJozUXqREVnctdZ2QZEwohNoIBiRr4KUi0j7ynQy0N-0CWK0CAHx1kJkoc4CpiM8ayzYJPN5kdttQhmPM6MynwdW8TTVKQNcTYH-ch5blnZb5LQRpBxVJ2fImUsZSF6i5CXD40lB8jJpEdGIW_4YAhLQ_a9XD5vukTA9UMQqt8V0ImOfRwJgpkW2ql6btQXjsYHM7fz_t7tkyUXmO--WPTJfjqd2HzhKmR64QXhAFs56Dzf3eL287t99Ad2P42Y
link.rule.ids 314,780,784,4502,24116,27924,27925,45585,45679
linkProvider Elsevier
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV07T8MwED7xkIAF8aY8PTDAEOomTuKOCIHKo10AKZvl2E6pVJKqTSX495ydRBUDQmKM45fO9ufvznc2wAULkeRnjHpd3kEFRQbKk7E2XmaYscd-PHWPwfQHUe-NPSZhsgS3TSyMdaussb_CdIfWdUq7lmZ7Mhq1X5w2EPuJhdww9JNlWEU2EOPUXr15eOoNFp4eYWVqwfyeLbAI5KkqcYmXlF65erzkty3qNwrqtqL7LdisOSS5qbq5DUsm34G1fn1KvgtjZ5kn1iZPLIsk5bssSfMUCi5ppJekLIjE_x9fZlyMtMuH0DIjMtfEfDr3WPzKzbycFpP30ZjMrOqs5fSLDHF_m48NGVqc3IO3-7vX255Xv6rgKUZ56UWaZ3HXGNNFXULzTqCQY0S-DFLGI-VL3clQhVM6iK1RCLm34jwLVRZQFdnrxoJ9WMmL3BwCoSzOtMx8FhjJ0lSlFKE1RQbMWGxo2m3BdSNIMakuzxALrzKUvLCSF9TeUIqSF0kLeCNu8WMWCAT4v4qeN8MjcIVYEcvcFPOZiH0WcUSaFhxUo7boiw3JRj539P9mz2G999p_Fs8Pg6dj2HCB-s7Z5QRWyuncnCJlKdOzekp-A8JX5JI
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Human+cell+line+that+differentiates+to+all+myeloid+lineages+and+expresses+neutrophil+secondary+granule+genes&rft.jtitle=Experimental+hematology&rft.au=Paul%2C+Cassandra+C&rft.au=Aly%2C+El-Sayed&rft.au=Lehman%2C+Jason+A&rft.au=Page%2C+Scott+M&rft.date=2000-12-01&rft.issn=0301-472X&rft.volume=28&rft.issue=12&rft.spage=1373&rft.epage=1380&rft_id=info:doi/10.1016%2FS0301-472X%2800%2900552-X&rft.externalDBID=n%2Fa&rft.externalDocID=10_1016_S0301_472X_00_00552_X
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0301-472X&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0301-472X&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0301-472X&client=summon