Insights into the Mutational Burden of Human Induced Pluripotent Stem Cells from an Integrative Multi-Omics Approach

• Published in: Cell reports (Cambridge) Vol. 24; no. 4; pp. 883 - 894
• Main Authors: D’Antonio, Matteo, Benaglio, Paola, Jakubosky, David, Greenwald, William W., Matsui, Hiroko, Donovan, Margaret K.R., Li, He, Smith, Erin N., D’Antonio-Chronowska, Agnieszka, Frazer, Kelly A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 24.07.2018
• Subjects: Cell Differentiation - physiology; Cells, Cultured; Cellular Reprogramming - genetics; epigenome; Humans; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - physiology; iPSCs; Mutation; Mutation Rate; RNA-seq; somatic mutations; stem cells; sublonal mutations; UV damage; whole genome sequencing; somatic mutations; whole genome sequencing; stem cells; iPSCs; epigenome; RNA-seq; sublonal mutations; UV damage

To understand the mutational burden of human induced pluripotent stem cells (iPSCs), we sequenced genomes of 18 fibroblast-derived iPSC lines and identified different classes of somatic mutations based on structure, origin, and frequency. Copy-number alterations affected 295 kb in each sample and st...