A Novel, Highly Sensitive Quantitative Polymerase Chain Reaction Assay for the Diagnosis of Subarachnoid and Ventricular Neurocysticercosis and for Assessing Responses to Treatment

• Published in: Clinical infectious diseases Vol. 70; no. 9; pp. 1875 - 1881
• Main Authors: O’Connell, Elise M, Harrison, Sarah, Dahlstrom, Eric, Nash, Theodore, Nutman, Thomas B
• Format: Journal Article
• Language: English
• Published: US Oxford University Press 15.04.2020
• Subjects: and Commentaries; Animals; Antigens, Helminth; Enzyme-Linked Immunosorbent Assay; Humans; Neurocysticercosis - diagnosis; Neurocysticercosis - drug therapy; Polymerase Chain Reaction; Reproducibility of Results; Sensitivity and Specificity; Taenia solium - genetics; subarachnoid disease; biomarker; PCR; neurocysticercosis; Taenia solium

Abstract Background Treatment of subarachnoid neurocysticercosis (NCC) is complicated, and assays that can guide treatment are not widely available. The reproducibility and scalability of molecular-based biomarkers would be of great use. Methods The Taenia solium genome was mined and primers and pro...