Inhibition of HDAC activity directly reprograms murine embryonic stem cells to trophoblast stem cells

• Published in: Developmental cell Vol. 59; no. 16; pp. 2101 - 2117.e8
• Main Authors: Huang, Boyan, Peng, Xing, Zhai, Xuzhao, Hu, Jie, Chen, Junyu, Yang, Suming, Huang, Qingpei, Deng, Enze, Li, Huanhuan, Barakat, Tahsin Stefan, Chen, Jiekai, Pei, Duanqing, Fan, Xiaoying, Chambers, Ian, Zhang, Man
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 19.08.2024
• Subjects: 2C-like cells; Acetylation - drug effects; Animals; blastoids; Butyric Acid - pharmacology; Cell Differentiation - drug effects; Cellular Reprogramming - drug effects; Epigenesis, Genetic; ESCs; Female; HDACi; Histone Deacetylase 1 - metabolism; Histone Deacetylase 2 - metabolism; Histone Deacetylase Inhibitors - pharmacology; Histone Deacetylases - metabolism; Histone Demethylases - metabolism; Mice; Mouse Embryonic Stem Cells - cytology; Mouse Embryonic Stem Cells - drug effects; Mouse Embryonic Stem Cells - metabolism; totipotency; transdifferentiation; Trophoblasts - cytology; Trophoblasts - drug effects; Trophoblasts - metabolism; TSCs; blastoids; 2C-like cells; TSCs; transdifferentiation; totipotency; HDACi; ESCs
• ISSN: 1534-5807; 1878-1551; 1878-1551
• DOI: 10.1016/j.devcel.2024.05.009

Embryonic stem cells (ESCs) can differentiate into all cell types of the embryonic germ layers. ESCs can also generate totipotent 2C-like cells and trophectodermal cells. However, these latter transitions occur at low frequency due to epigenetic barriers, the nature of which is not fully understood....