Nuclear proteasomal degradation of Saccharomyces cerevisiae inorganic pyrophosphatase Ipp1p, a nucleocytoplasmic protein whose stability depends on its subcellular localization

• Published in: Biochimica et biophysica acta. Molecular cell research Vol. 1866; no. 6; pp. 1019 - 1033
• Main Authors: Serrano-Bueno, Gloria, Madroñal, Juan Manuel, Manzano-López, Javier, Muñiz, Manuel, Pérez-Castiñeira, José Román, Hernández, Agustín, Serrano, Aurelio
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2019
• Subjects: Cell nucleus; Inorganic pyrophosphatase; Nucleocytoplasmic transport; Protein degradation; Ubiquitin-proteasome system; NESHV; Protein degradation; Cell nucleus; PPi; sPPase; GFP; Inorganic pyrophosphatase; NLS; NLSe; NLSSV; HA; Nucleocytoplasmic transport; Ubiquitin-proteasome system; NES

Inorganic pyrophosphate (PPi) is an abundant by-product of cellular metabolism. PPi-producing reactions take place in the nucleus concurrently with reactions that use PPi as a substrate. Saccharomyces cerevisiae possesses two soluble pyrophosphatases (sPPases): Ipp1p, an essential and allegedly cyto...