The Mycobacterial LexA/RecA-Independent DNA Damage Response Is Controlled by PafBC and the Pup-Proteasome System

• Published in: Cell reports (Cambridge) Vol. 23; no. 12; pp. 3551 - 3564
• Main Authors: Müller, Andreas U., Imkamp, Frank, Weber-Ban, Eilika
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 19.06.2018
• Subjects: Amino Acid Sequence; bacterial proteasome; Bacterial Proteins - metabolism; Base Sequence; Clp gene regulator ClgR; DNA Damage; DNA damage response; DNA Repair - drug effects; DNA Repair - genetics; DNA, Bacterial - genetics; Gene Expression Regulation, Bacterial - drug effects; LexA/RecA-independent pathway; Mitomycin - pharmacology; Mycobacterium - drug effects; Mycobacterium - genetics; Mycobacterium - metabolism; Mycobacterium smegmatis; Nucleotide Motifs - genetics; PafBC regulon; Promoter Regions, Genetic; Proteasome Endopeptidase Complex - metabolism; pupylation; Rec A Recombinases - chemistry; Rec A Recombinases - metabolism; RecA-NDp promoter; Recombination, Genetic - genetics; Regulon - genetics; Serine Endopeptidases - metabolism; Stress, Physiological - drug effects; Stress, Physiological - genetics; Trans-Activators - metabolism; Transcriptome - drug effects; Transcriptome - genetics; Up-Regulation - drug effects; Up-Regulation - genetics; PafBC regulon; pupylation; Clp gene regulator ClgR; bacterial proteasome; DNA damage response; Mycobacterium smegmatis; RecA-NDp promoter; LexA/RecA-independent pathway

Mycobacteria exhibit two DNA damage response pathways: the LexA/RecA-dependent SOS response and a LexA/RecA-independent pathway. Using a combination of transcriptomics and genome-wide binding site analysis, we demonstrate that PafBC (proteasome accessory factor B and C), encoded in the Pup-proteasom...