Association between the expression of TLR4, TLR2, and MyD88 with low-grade chronic inflammation in individuals with metabolically healthy obesity

• Published in: Molecular biology reports Vol. 50; no. 5; pp. 4723 - 4728
• Main Authors: Guerrero-Romero, Fernando, Castellanos-Juárez, Francisco X., Salas-Pacheco, Jose M., Morales-Gurrola, Francisco G., Salas-Leal, Alma Cristina, Simental-Mendía, Luis E.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.05.2023; Springer Nature B.V
• Subjects: Adaptor Proteins, Signal Transducing - metabolism; alcohol drinking; Animal Anatomy; Animal Biochemistry; Biomedical and Life Sciences; Blood pressure; Body Mass Index; Cardiovascular diseases; Cholesterol; Cross-Sectional Studies; diabetes; Diabetes mellitus; Female; High density lipoprotein; high density lipoprotein cholesterol; Histology; Humans; Hyperglycemia; Hypertension; Hypertension - metabolism; Hypertriglyceridemia; Inflammation; Inflammation - genetics; Life Sciences; Liver diseases; Morphology; MyD88 protein; Myeloid Differentiation Factor 88 - genetics; Myeloid Differentiation Factor 88 - metabolism; Obesity; Obesity, Metabolically Benign; phenotype; Phenotypes; Physical activity; pregnancy; regression analysis; risk; Risk Factors; Sexual intercourse; Short Communication; Thyroid cancer; Thyroid diseases; TLR2 protein; TLR4 protein; Toll-Like Receptor 2 - genetics; Toll-Like Receptor 2 - metabolism; Toll-like receptors; Obesity; Inflammation; TLR; Metabolically healthy obesity; MyD88
• DOI: 10.1007/s11033-023-08338-z
• ISSN: 0301-4851; 1573-4978

Background Among the Toll-like receptors (TLR) that are dependent of myeloid response protein (MyD88), the TLR4 and TLR2 are directly associated with low-grade chronic inflammation; however, they are not been investigated in subjects with metabolically healthy obesity (MHO). Thus, the objective of this study was to determine the association between the expression of TLR4, TLR2, and MyD88 with low-grade chronic inflammation in individuals with MHO. Methods and results Men and women with obesity aged 20 to 55 years were enrolled in a cross-sectional study. Individuals with MHO were allocated into the groups with and without low-grade chronic inflammation. Pregnancy, smoking, alcohol consumption, intense physical activity or sexual intercourse in the previous 72 h, diabetes, high blood pressure, cancer, thyroid disease, acute or chronic infections, renal impairment, and hepatic diseases, were exclusion criteria. The MHO phenotype was defined by a body mass index (BMI ≥ 30 kg/m 2 ) plus one or none of the following cardiovascular risk factors: hyperglycemia, elevated blood pressure, hypertriglyceridemia, and low high-density lipoprotein cholesterol. A total of 64 individuals with MHO were enrolled and allocated into the groups with (n = 37) and without (n = 27) inflammation. The multiple logistic regression analysis indicated that TLR2 expression is significantly associated with inflammation in individuals with MHO. In the subsequent analysis adjusted by BMI, TLR2 expression remained associated with inflammation in individuals with MHO. Conclusion Our results suggest that overexpression of TLR2, but not TLR4 and MyD88, is associated with low-grade chronic inflammation in subjects with MHO.