Detection of Low-abundance Point Mutations by Competitive Strand Assisted Endonuclease Ⅳ Signal Amplification System

• Published in: Current medical science Vol. 37; no. 5; pp. 803 - 806
• Main Author: 熊飞;刘传珍;李万强;董自强;张杰
• Format: Journal Article
• Language: English
• Published: Wuhan Huazhong University of Science and Technology 01.10.2017; Department of Urology, Renmin Hospital of Wuhan University, Wuhan 430060, China%Department of Urology, Yichang Central People's Hospital, the first College of Clinical Medical Science, China Three Gorges University, Yichang 443003, China
• Subjects: Medicine; Medicine & Public Health; competitive DNA probe; low-abundance point mutation; melting temperature discrimination; endonuclease IV; endonuclease
• ISSN: 1672-0733; 2096-5230; 1993-1352; 1993-1352; 2523-899X
• DOI: 10.1007/s11596-017-1808-7

Genetic mutations are important molecular biomarkers for cancer diagnosis and surveillance. Therefore, the development of methods for mutation detection characterized with straightforward, highly specific and sensitive to low-level mutations within various sequence contexts is extremely needed. Alth...