5′ exon interactions within the human spliceosome establish a framework for exon junction complex structure and assembly

• Published in: Genes & development Vol. 16; no. 21; pp. 2778 - 2791
• Main Authors: Reichert, Vienna L., Le Hir, Hervé, Jurica, Melissa S., Moore, Melissa J.
• Format: Journal Article
• Language: English
• Published: United States Cold Spring Harbor Laboratory Press 01.11.2002
• Subjects: Exons - genetics; Humans; Research Paper; Ribonucleoproteins - genetics; RNA Precursors - genetics; RNA Splicing; RNA, Messenger - genetics; Spliceosomes - genetics

A general consequence of pre-mRNA splicing is the stable deposition of several proteins 20–24 nucleotides (nt) upstream of exon–exon junctions on spliced mRNAs. This exon junction complex (EJC) contains factors involved in mRNA export, cytoplasmic localization, and nonsense-mediated mRNA decay. Here...