Cloning, expression, and purification of porcine pancreatic phospholipase A2 and mutants

• Published in: Methods in enzymology Vol. 197; p. 214
• Main Authors: Verheij, H M, de Haas, G H
• Format: Journal Article
• Language: English
• Published: United States 1991
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Chromatography, DEAE-Cellulose - methods; Cloning, Molecular - methods; DNA - genetics; DNA - isolation & purification; Escherichia coli - genetics; Gene Expression; Molecular Sequence Data; Mutagenesis; Pancreas - enzymology; Phospholipases A - genetics; Phospholipases A - isolation & purification; Phospholipases A2; Recombinant Proteins - isolation & purification; Saccharomyces cerevisiae - genetics; Swine