Integrin αvβ3 mediates the synergetic regulation of core-binding factor α1 transcriptional activity by gravity and insulin-like growth factor-1 through phosphoinositide 3-kinase signaling

• Published in: Bone (New York, N.Y.) Vol. 69; pp. 126 - 132
• Main Authors: Dai, Zhongquan, Guo, Feima, Wu, Feng, Xu, Hongjie, Yang, Chao, Li, Jinqiao, Liang, Peilong, Zhang, Hongyu, Qu, Lina, Tan, Yingjun, Wan, Yumin, Li, Yinghui
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.12.2014; Elsevier
• Subjects: 3T3 Cells; Animals; Biological and medical sciences; Blotting, Western; Bone loss; Bone Remodeling - physiology; Cbfa1; Core Binding Factor Alpha 1 Subunit - metabolism; Diseases of the osteoarticular system; Fundamental and applied biological sciences. Psychology; Gravity; Gravity, Altered - adverse effects; IGF-1; Immunoprecipitation; Insulin-Like Growth Factor I - metabolism; Integrin alphaVbeta3 - metabolism; Integrin αvβ3; Medical sciences; Mice; Orthopedics; Osteoporosis. Osteomalacia. Paget disease; Phosphatidylinositol 3-Kinases - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Small Interfering; Signal Transduction - drug effects; Space Flight; Synergetic regulation; Transfection; Traumas. Diseases due to physical agents; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Weightlessness - adverse effects; CCB; Col1a1; IRS1; ALP; Cbfa1; IGF-1; Synergetic regulation; SMG; EGFP; co-IP; Integrin αvβ3; OSE2; PI3K; Bone loss; HG; Gravity; hypergravity; co-immunoprecipitation; cell culture box; core-binding factor alpha 1; alkaline phosphatase; Type I Collagen alpha 1 chain; Osteoblast-Specific Element 2; insulin receptor substrate 1; enhanced green fluorescent protein; Insulin-like growth factor 1; simulated microgravity; phosphoinositide 3-kinase; Enzyme; Transferases; Diseases of the osteoarticular system; Insulin like growth factor 1; Integrin; Kinase; Morphology; Orthopedics; Osteopenia

Mechanical stimulation and biological factors coordinately regulate bone development and regeneration; however, the underlying mechanisms are poorly understood. Microgravity induces bone loss, which may be partly related to the development of resistance to local cytokines, including insulin-like gro...