Yeast Secretes High Amounts of Human Calreticulin without Cellular Stress

• Published in: Current issues in molecular biology Vol. 44; no. 5; pp. 1768 - 1787
• Main Authors: Zinkevičiūtė, Rūta, Ražanskas, Raimundas, Kaupinis, Algirdas, Macijauskaitė, Neringa, Čiplys, Evaldas, Houen, Gunnar, Slibinskas, Rimantas
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 19.04.2022; MDPI AG
• Subjects: 2DE; calreticulin; LC-MSE; proteomics; secretion; yeast; 2DE; secretion; proteomics; recombinant protein; calreticulin; yeast; LC-MSE; cellular stress

The ER chaperone calreticulin (CALR) also has extracellular functions and can exit the mammalian cell in response to various factors, although the mechanism by which this takes place is unknown. The yeast efficiently secretes human CALR, and the analysis of this process in yeast could help to clarif...