Getting the glycosylation right: Implications for the biotechnology industry

Glycosylation is the most extensive of all the posttranslational modifications, and has important functions in the secretion, antigenicity and clearance of glycoproteins. In recent years major advances have been made in the cloning of glycosyltransferase enzymes, in understanding the varied biologic...

Full description

Saved in:
Bibliographic Details
Published inNature biotechnology Vol. 14; no. 8; pp. 975 - 981
Main Authors Jenkins, Nigel, Parekh, Raj B, James, David C
Format Journal Article
LanguageEnglish
Published United States 01.08.1996
Subjects
Animals
Biotechnology
Cell Culture Techniques
Cloning, Molecular
Glycoproteins - chemistry
Glycosylation
Humans
Oligosaccharides - chemistry
Online AccessGet full text

Cover

More Information
Summary:Glycosylation is the most extensive of all the posttranslational modifications, and has important functions in the secretion, antigenicity and clearance of glycoproteins. In recent years major advances have been made in the cloning of glycosyltransferase enzymes, in understanding the varied biological functions of carbohydrates, and in the accurate analysis of glycoprotein heterogeneity. In this review we discuss the impact of these advances on the choice of a recombinant host cell line, in optimizing cell culture processes, and in choosing the appropriate level of glycosylation analysis for each stage of product development.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
ObjectType-Review-3
content type line 23
ObjectType-Feature-3
ObjectType-Review-1
ISSN:1087-0156
1546-1696
DOI:10.1038/nbt0896-975