Differentiation of cucumber mosaic virus isolates using the polymerase chain reaction

• Published in: Journal of general virology Vol. 73; no. 8; pp. 2099 - 2103
• Main Authors: Rizos, Helen, Gunn, Linda V, Pares, Ray D, Gillings, Michael R
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.08.1992; Society for General Microbiology
• Subjects: Base Sequence; Biological and medical sciences; Capsid - genetics; Cloning, Molecular; Fundamental and applied biological sciences. Psychology; Microbiology; Molecular Sequence Data; Mosaic Viruses - classification; Mosaic Viruses - genetics; Oligodeoxyribonucleotides - genetics; Plants - microbiology; Polymerase Chain Reaction; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; Restriction Mapping; RNA, Viral - genetics; Virology; Virus; Polymerase chain reaction; Plant pathogen; DNA; Cucumber mosaic virus; Restriction fragment; Isolate; Cucumovirus; Identification; Method
• DOI: 10.1099/0022-1317-73-8-2099
• ISSN: 0022-1317

1 School of Biological Sciences, Macquarie University, North Ryde, NSW 2109 and 2 Biological and Chemical Research Institute, Rydalmere, NSW 2116, Australia A procedure based on the polymerase chain reaction (PCR) has been developed to classify cucumber mosaic cucumovirus (CMV) isolates accurately into two subgroups. Two CMV-specific primers that flank the CMV capsid protein gene were used to amplify a DNA fragment of approximately 870 bp. Restriction enzyme analysis of this fragment produces distinct restriction patterns that assign the CMV isolate into one of two subgroups. These two restriction groups correlate with the previously established CMV subgroupings; this PCR-based method may provide a simple alternative to the serological assays used for typing CMV isolates. Received 24 January 1992; accepted 16 April 1992.