Gene Targeting and Cloning in Pigs Using Fetal Liver Derived Cells

• Published in: The Journal of surgical research Vol. 171; no. 2; pp. e223 - e229
• Main Authors: Waghmare, Sanjeev K., Ph.D, Estrada, Jose, D.V.M., Ph.D, Reyes, Luz, Ph.D, Li, Ping, Ph.D, Ivary, Bess, B.S, Sidner, Richard A., Ph.D, Burlak, Chris, Ph.D, Tector, A. Joseph, M.D., Ph.D
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2011
• Subjects: Animals; Animals, Genetically Modified - genetics; Cell Culture Techniques - methods; cloning; Cloning, Organism - methods; Embryonic Stem Cells - cytology; Embryonic Stem Cells - physiology; Feasibility Studies; Female; fetal liver-derived cells; Fetus - cytology; Fibroblasts - cytology; Fibroblasts - physiology; Gene Knockout Techniques; Gene Targeting - methods; genetic engineering; Genetic Engineering - methods; Homozygote; Liver - cytology; Loss of Heterozygosity - genetics; Male; Nuclear Transfer Techniques; Pregnancy; Surgery; Swine - genetics; Tail - cytology; Transplantation, Heterologous; xenotransplantation; fetal liver-derived cells; xenotransplantation; cloning; genetic engineering

Background Since there are no pig embryonic stem cells, pig genetic engineering is done in fetal fibroblasts that remain totipotent for only 3 to 5 wk. Nuclear donor cells that remain totipotent for longer periods of time would facilitate complicated genetic engineering in pigs. The goal of this stu...