Mechanistic screening of reproductive toxicity in a novel 3D testicular co-culture model shows significant impairments following exposure to low-dibutyl phthalate concentrations

• Published in: Archives of toxicology Vol. 98; no. 8; pp. 2695 - 2709
• Main Authors: Almamoun, Radwa, Pierozan, Paula, Karlsson, Oskar
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2024; Springer Nature B.V
• Subjects: Animals; Animals, Newborn; Antiandrogen; Biomedical and Life Sciences; Biomedicine; Biosynthesis; Cell culture; Cholesterol Side-Chain Cleavage Enzyme - metabolism; Coculture Techniques; Contaminants; Dibutyl phthalate; Dibutyl Phthalate - toxicity; Dose-Response Relationship, Drug; Drug development; Environmental Health; Environmental Pollutants - toxicity; Exposure; Germ cells; High-throughput screening; Leydig cells; Leydig Cells - drug effects; Leydig Cells - metabolism; Male; Mechanistic toxicology; Mice; Minipuberty; n-Butyl phthalate; N-Cadherin; Neonates; Occupational Medicine/Industrial Medicine; Pharmacology/Toxicology; Phthalates; Proteins; Reproduction - drug effects; Reproductive health; Reproductive toxicity; Reproductive Toxicology; Risk assessment; RNA-binding protein; Screening; Sertoli cells; Sertoli Cells - drug effects; Sertoli Cells - metabolism; Sox9 protein; Spermatogenesis; Testes; Testis - drug effects; Testis - metabolism; Testosterone; Testosterone - metabolism; Three dimensional models; Toxicants; Toxicity; Zonula occludens-1 protein; Testosterone; Antiandrogen; Minipuberty; Spermatogenesis; Reproductive toxicity; Mechanistic toxicology

To improve the mechanistic screening of reproductive toxicants in  chemical-risk assessment and drug development, we have developed a three-dimensional (3D) heterogenous testicular co-culture model from neonatal mice. Di-n-butyl phthalate (DBP), an environmental contaminant that can affect reproduct...