Cloning and expression of a mammalian Na+/amino acid cotransporter with sequence similarity to Na+/glucose cotransporters
We describe the full-length sequence and functional expression of a cDNA cloned from LLC-PK1 cells, which appears to encode a mammalian Na(+)-dependent neutral amino acid transporter with properties characteristic of system A. This sequence, designated SAAT1, is 76% identical and 89% similar in amin...
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Published in | The Journal of biological chemistry Vol. 268; no. 3; pp. 1509 - 1512 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Bethesda, MD
American Society for Biochemistry and Molecular Biology
25.01.1993
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Abstract | We describe the full-length sequence and functional expression of a cDNA cloned from LLC-PK1 cells, which appears to encode
a mammalian Na(+)-dependent neutral amino acid transporter with properties characteristic of system A. This sequence, designated
SAAT1, is 76% identical and 89% similar in amino acid sequence to the Na(+)-dependent glucose transporter SGLT1 of the same
species. A leucine zipper region was detected in both SAAT1 and SGLT1. The message for SAAT1 was a single 2.4-kilobase species
in kidney, but mRNA species of 2.4 and 3.7 kilobases were observed in LLC-PK1 cells as well as in intestine. Transcripts were
also found in spleen, liver, and muscle. Expression of SAAT1 in COS-7 cells resulted in increased levels of Na(+)-dependent
uptake of 2-(methylamino)isobutyric acid, a specific substrate for the system A amino acid transporter. Uptake due to cDNA
expression was inhibited by a range of amino acids that are transported by system A and exhibited a km of 0.8 +/- 0.2 mM.
These results suggest that the system A amino acid transporter is closely related to the Na+/glucose transporter SGLT1. |
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AbstractList | We describe the full-length sequence and functional expression of a cDNA cloned from LLC-PK1 cells, which appears to encode a mammalian Na(+)-dependent neutral amino acid transporter with properties characteristic of system A. This sequence, designated SAAT1, is 76% identical and 89% similar in amino acid sequence to the Na(+)-dependent glucose transporter SGLT1 of the same species. A leucine zipper region was detected in both SAAT1 and SGLT1. The message for SAAT1 was a single 2.4-kilobase species in kidney, but mRNA species of 2.4 and 3.7 kilobases were observed in LLC-PK1 cells as well as in intestine. Transcripts were also found in spleen, liver, and muscle. Expression of SAAT1 in COS-7 cells resulted in increased levels of Na(+)-dependent uptake of 2-(methylamino)isobutyric acid, a specific substrate for the system A amino acid transporter. Uptake due to cDNA expression was inhibited by a range of amino acids that are transported by system A and exhibited a km of 0.8 +/- 0.2 mM. These results suggest that the system A amino acid transporter is closely related to the Na+/glucose transporter SGLT1. We describe the full-length sequence and functional expression of a cDNA cloned from LLC-PK1 cells, which appears to encode a mammalian Na(+)-dependent neutral amino acid transporter with properties characteristic of system A. This sequence, designated SAAT1, is 76% identical and 89% similar in amino acid sequence to the Na(+)-dependent glucose transporter SGLT1 of the same species. A leucine zipper region was detected in both SAAT1 and SGLT1. The message for SAAT1 was a single 2.4-kilobase species in kidney, but mRNA species of 2.4 and 3.7 kilobases were observed in LLC-PK1 cells as well as in intestine. Transcripts were also found in spleen, liver, and muscle. Expression of SAAT1 in COS-7 cells resulted in increased levels of Na(+)-dependent uptake of 2-(methylamino)isobutyric acid, a specific substrate for the system A amino acid transporter. Uptake due to cDNA expression was inhibited by a range of amino acids that are transported by system A and exhibited a km of 0.8 +/- 0.2 mM. These results suggest that the system A amino acid transporter is closely related to the Na+/glucose transporter SGLT1. |
Author | J E Lever C T Kong S F Yet |
Author_xml | – sequence: 1 givenname: C T surname: Kong fullname: Kong, C T organization: Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77225 – sequence: 2 givenname: S F surname: Yet fullname: Yet, S F – sequence: 3 givenname: J E surname: Lever fullname: Lever, J E |
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Keywords | Vertebrata Mammalia Sodium Aminoacid Primary structure Coupled transport Glucose Gene expression Carrier protein |
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Snippet | We describe the full-length sequence and functional expression of a cDNA cloned from LLC-PK1 cells, which appears to encode
a mammalian Na(+)-dependent neutral... We describe the full-length sequence and functional expression of a cDNA cloned from LLC-PK1 cells, which appears to encode a mammalian Na(+)-dependent neutral... |
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SubjectTerms | Amino Acid Sequence Amino Acid Transport Systems Analytical, structural and metabolic biochemistry Animals Base Sequence Binding and carrier proteins Biological and medical sciences Carrier Proteins - chemistry Carrier Proteins - genetics Cell Line Cloning, Molecular DNA - chemistry DNA Probes Fundamental and applied biological sciences. Psychology Gene Expression Glycosylation Humans Kidney - chemistry Leucine Zippers Molecular Sequence Data Monosaccharide Transport Proteins - chemistry Proteins Rabbits RNA, Messenger - analysis Sequence Homology, Amino Acid Sodium - pharmacology |
Title | Cloning and expression of a mammalian Na+/amino acid cotransporter with sequence similarity to Na+/glucose cotransporters |
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