Liquid chromatography in pharmaceutical analysis VIII: determination of isoniazid and acetyl derivative in plasma and urine samples

• Published in: Journal of pharmaceutical sciences Vol. 66; no. 6; p. 813
• Main Authors: Saxena, S J, Stewart, J T, Honigberg, I L, Washington, J G, Keene, G R
• Format: Journal Article
• Language: English
• Published: United States 01.06.1977
• Subjects: Acetylation; Chromatography, High Pressure Liquid; Humans; Isoniazid - analysis; Isoniazid - blood; Isoniazid - urine; Methods

Parameters are described for the qualitative and quantitative analysis of a mixture of isoniazid and its acetyl derivative. The compounds are chromatographed on an octadecylsilane column, using absolute methanol-distilled water (60:40) at pH 2.5 containing 0.01 M dioctyl sodium sulfosuccinate. The f...