Liquid chromatography in pharmaceutical analysis VIII: determination of isoniazid and acetyl derivative in plasma and urine samples

Parameters are described for the qualitative and quantitative analysis of a mixture of isoniazid and its acetyl derivative. The compounds are chromatographed on an octadecylsilane column, using absolute methanol-distilled water (60:40) at pH 2.5 containing 0.01 M dioctyl sodium sulfosuccinate. The f...

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Bibliographic Details
Published inJournal of pharmaceutical sciences Vol. 66; no. 6; p. 813
Main Authors Saxena, S J, Stewart, J T, Honigberg, I L, Washington, J G, Keene, G R
Format Journal Article
LanguageEnglish
Published United States 01.06.1977
Subjects
Acetylation
Chromatography, High Pressure Liquid
Humans
Isoniazid - analysis
Isoniazid - blood
Isoniazid - urine
Methods
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Summary:Parameters are described for the qualitative and quantitative analysis of a mixture of isoniazid and its acetyl derivative. The compounds are chromatographed on an octadecylsilane column, using absolute methanol-distilled water (60:40) at pH 2.5 containing 0.01 M dioctyl sodium sulfosuccinate. The flow rate was 2.0 ml/min (2500 psig). The separation and quantification are applicable to plasma and urine samples. The determination in each biological fluid can be achieved in approximately 90 min with percentage accuracies for isoniazid of 5.25 and 7.45 and for the acetyl derivative of 4.47 and 1.56 in plasma and urine, respectively.
ISSN:0022-3549
DOI:10.1002/jps.2600660618