Treatment of neonatal mice with Flt3 ligand leads to changes in dendritic cell subpopulations associated with enhanced IL‐12 and IFN‐α production

• Published in: European Journal of Immunology Vol. 34; no. 7; pp. 1849 - 1860
• Main Authors: Vollstedt, Sabine, O'Keeffe, Meredith, Odermatt, Bernhard, Beat, Ryf, Glanzmann, Bettina, Riesen, Matthias, Shortman, Ken, Suter, Mark
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY‐VCH Verlag 01.07.2004
• Subjects: Animals; Animals, Newborn; Cell Differentiation - drug effects; Cell Lineage - drug effects; Cytokine production; DC subpopulations; Dendritic Cells - cytology; Dendritic Cells - drug effects; Dendritic Cells - immunology; Dendritic Cells - metabolism; Female; Flow Cytometry; Flt3 ligand; Interferon-alpha - biosynthesis; Interleukin-12 - biosynthesis; Lymphocyte Activation - drug effects; Lymphocyte Culture Test, Mixed; Male; Membrane Proteins - pharmacology; Mice; Mice, Inbred C57BL; Neonatal mice; Phenotype; T-Lymphocytes - immunology

Treatment with the hematopoietic growth factor Flt3 ligand (FL) increases DC numbers in neonatal mice and enhances their resistance against intracellular pathogens. Flow cytometric analysis showed the presence of conventional DC (cDC) and plasmacytoid pre‐DC (pDC) in neonatal spleens from untreated...