A guide to assessing cellular senescence in vitro and in vivo

• Published in: The FEBS journal Vol. 288; no. 1; pp. 56 - 80
• Main Authors: González‐Gualda, Estela, Baker, Andrew G., Fruk, Ljiljana, Muñoz‐Espín, Daniel
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.01.2021
• Subjects: ageing; Aging; Aging - genetics; Aging - metabolism; Animals; Animals, Genetically Modified; assessment; Biomarkers; Biomarkers - metabolism; Cell cycle; cell cycle checkpoints; Cell Cycle Checkpoints - genetics; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; Cell Proliferation; cell senescence; Cell Shape - genetics; Cells, Cultured; cellular senescence; Cellular Senescence - genetics; Combinatorial analysis; Cytokines - genetics; Cytokines - immunology; detection; Diagnostic systems; DNA Damage; Embryogenesis; Embryonic growth stage; Genetic Loci; Heterochromatin - chemistry; Heterochromatin - metabolism; Heterogeneity; Humans; Lamin Type B - deficiency; Lamin Type B - genetics; Lysosomes - metabolism; Mitochondria - genetics; Mitochondria - metabolism; Mitochondria - pathology; phenotype; Phenotypes; Senescence; therapeutics; Wound healing; assessment; ageing; detection; cellular senescence; biomarkers

Cellular senescence is a complex cell response to damage and stress that can impact the surrounding tissue. Senescent cells no longer proliferate and undergo particular phenotypic changes. However, their phenotype can be very variable, and identifying senescence remains a challenge. In this review,...