Exploring the effect of library preparation on RNA sequencing experiments

• Published in: Genomics (San Diego, Calif.) Vol. 111; no. 6; pp. 1752 - 1759
• Main Authors: Wang, Lei, Felts, Sara J., Van Keulen, Virginia P., Pease, Larry R., Zhang, Yuji
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2019
• Subjects: B-lymphocytes; blood sampling; cDNA libraries; complementary DNA; Cryopreservation; Gene Expression Profiling; Gene Library; genes; High-Throughput Nucleotide Sequencing; Humans; Library storage time; lincRNA; patients; Quantity of input RNA; RNA; RNA-seq; sequence analysis; Sequence Analysis, RNA; storage time; surveys; transcription (genetics); Transcriptome; Quantity of input RNA; lincRNA; RNA-seq; Cryopreservation; Library storage time

RNA sequencing (RNA-seq) has become the widely preferred choice for surveying the genome-wide transcriptome complexity in many organisms. However, the broad adaptation of this methodology into the clinic still needs further evaluation of potential effect of sample preparation factors on its analytic...