Analysis of N-linked Oligosaccharides Released from Glycoproteins Separated by Two-Dimensional Gel Electrophoresis

• Published in: Analytical biochemistry Vol. 284; no. 1; pp. 49 - 59
• Main Authors: Charlwood, Joanne, Skehel, J.Mark, Camilleri, Patrick
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.08.2000
• Subjects: alpha-Fetoproteins - chemistry; Animals; Chromatography, High Pressure Liquid; Chromatography, Liquid; Electrophoresis, Gel, Two-Dimensional - methods; Electrophoresis, Polyacrylamide Gel; Glycoproteins - analysis; Glycoproteins - blood; Glycoproteins - metabolism; Hydrogen-Ion Concentration; Indicators and Reagents - pharmacology; Mice; Oligosaccharides - analysis; Oligosaccharides - blood; Polysaccharides - metabolism; Polyvinyls - pharmacology; Proflavine - analogs & derivatives; Proflavine - pharmacology; Rosaniline Dyes - pharmacology; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Protocols have been developed for the characterization of carbohydrate covalently attached (N-linked) to an asparagine residue in glycoproteins, after separation by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Mixtures of proteins (each at a level from 0.5 to 50 μg) were resolved i...