Single-cell RNA sequencing deconvolutes the in vivo heterogeneity of human bone marrow-derived mesenchymal stem cells

• Published in: International journal of biological sciences Vol. 17; no. 15; pp. 4192 - 4206
• Main Authors: Wang, Zun, Li, Xiaohua, Yang, Junxiao, Gong, Yun, Zhang, Huixi, Qiu, Xiang, Liu, Ying, Zhou, Cui, Chen, Yu, Greenbaum, Jonathan, Cheng, Liang, Hu, Yihe, Xie, Jie, Yang, Xucheng, Li, Yusheng, Schiller, Martin R., Chen, Yiping, Tan, Lijun, Tang, Si-Yuan, Shen, Hui, Xiao, Hong-Mei, Deng, Hong-Wen
• Format: Journal Article
• Language: English
• Published: Australia Ivyspring International Publisher Pty Ltd 01.01.2021; Ivyspring International Publisher
• Subjects: Adipocytes; Aged; Aged, 80 and over; Angiogenesis; Animals; Annotations; Antibodies; Antigens; Biomarkers; Biomedical materials; Blood diseases; Bone Density; Bone marrow; Bone Marrow Cells - classification; Bone Marrow Cells - metabolism; Bone turnover; Cartilage; CD56 Antigen - genetics; CD56 Antigen - metabolism; Cell Differentiation; Chondrocytes; Chondrocytes - physiology; Cloning; Cluster Analysis; Codification; Differentiation; Female; Fibroblasts; Gene expression; Gene Expression Regulation; Gene sequencing; Glial stem cells; Hemopoiesis; Heterogeneity; Homeostasis; Humans; In vivo methods and tests; Joint replacement surgery; Laboratories; Leukocytes (mononuclear); Male; Mesenchymal stem cells; Mesenchymal Stem Cells - metabolism; Mice; Mice, Inbred C57BL; Muscle Development; Myocytes; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - metabolism; Osteoblasts; Osteogenesis; Osteoprogenitor cells; Population; Precursors; Receptors, Nerve Growth Factor - genetics; Receptors, Nerve Growth Factor - metabolism; Research Paper; Ribonucleic acid; RNA; Sequence Analysis, RNA; Single-Cell Analysis - methods; Stem cells; Stromal cells; Subpopulations; Transcription; Transcriptomes; Vertebrae; United States > US; mesenchymal stem cell (MSC); adipogenesis; single-cell RNA sequencing (scRNA-seq); chondrogenesis; osteogenesis; bone marrow

Bone marrow-derived mesenchymal stem cells (BM-MSCs) are multipotent stromal cells that have a critical role in the maintenance of skeletal tissues such as bone, cartilage, and the fat in bone marrow. In addition to providing microenvironmental support for hematopoietic processes, BM-MSCs can differ...