Over-expression of a fungal NADP(H)-dependent glutamate dehydrogenase PcGDH improves nitrogen assimilation and growth quality in rice

• Published in: Molecular breeding Vol. 34; no. 2; pp. 335 - 349
• Main Authors: Zhou, Yanbiao, Liu, Hong, Zhou, Xiangcheng, Yan, Yuanzhu, Du, Changqing, Li, Yixing, Liu, Derong, Zhang, Caisheng, Deng, Xiaolong, Tang, Dongying, Zhao, Xiaoying, Zhu, Yonghua, Lin, Jianzhong, Liu, Xuanming
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.08.2014; Springer Netherlands; Springer Nature B.V
• Subjects: Affinity; Amination; Amino acids; Ammonium; Assimilation; Biological assimilation; Biomedical and Life Sciences; Biotechnology; carbon; Chlorophyll; Crop yield; Deamination; Dehydrogenase; Dehydrogenases; Fungi; genes; Glutamate dehydrogenase; Glutamate-ammonia ligase; glutamic acid; Glutamine; Glutelin; Grain; grain yield; Hydroponics; inflorescences; Ketoglutaric acid; Life Sciences; metabolism; Molecular biology; NADP; Nitrogen; nitrogen content; Oryza sativa; Overexpression; phenylpropanolamine; Plant biology; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Pleurotus; Prolamines; Proteins; Rice; Seedlings; Seeds; Shoots; Transgenic plants; Weight; NADP(H)-dependent glutamate dehydrogenase; Ammonium assimilation; Rice

Glutamate dehydrogenase (GDH) tends to have a lower affinity for ammonium than glutamine synthetase (GS) in higher plants. Consequently, nitrogen is mostly assimilated as ammonium by the GS/glutamate synthase pathway which requires 2-oxoglutarate (2-OG) as carbon skeletons. In contrast, the NADP(H)-...