An integrated-molecular-beacon based multiple exponential strand displacement amplification strategy for ultrasensitive detection of DNA methyltransferase activity

• Published in: Chemical science (Cambridge) Vol. 10; no. 8; pp. 2290 - 2297
• Main Authors: Cui, Yun-Xi, Feng, Xue-Nan, Wang, Ya-Xin, Pan, Hui-Yu, Pan, Hua, Kong, De-Ming
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 28.02.2019
• Subjects: Amplification; Chemistry; Deoxyribonucleic acid; DNA; DNA methylation; Enlargement; Fluorescence; Gene expression; Oligonucleotides; Substrates

DNA methylation is a significant epigenetic mechanism involving processes of transferring a methyl group onto cytosine or adenine. Such DNA modification catalyzed by methyltransferase (MTase) plays important roles in the modulation of gene expression and other cellular activities. Herein, we develop...