Biochemical Analysis of the Modular Enzyme Inosine 5′-Monophosphate Dehydrogenase

• Published in: Protein expression and purification Vol. 17; no. 2; pp. 282 - 289
• Main Authors: Nimmesgern, Elmar, Black, James, Futer, Olga, Fulghum, John R., Chambers, Stephen P., Brummel, Christopher L., Raybuck, Scott A., Sintchak, Michael D.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.1999
• Subjects: Animals; Bacteriophage T7 - genetics; Binding Sites; Circular Dichroism; Cricetinae; Electrophoresis, Polyacrylamide Gel; Histidine - metabolism; Humans; IMP Dehydrogenase - chemistry; Kinetics; Middle Aged; Molecular Probes - metabolism; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Fusion Proteins - biosynthesis; Transcription, Genetic

Two prominent domains have been identified in the X-ray crystal structure of inosine-5′-monophosphate dehydrogenase (IMPDH), a core domain consisting of an α/β barrel which contains the active site and an inserted subdomain whose structure is less well defined. The core domain encompassing amino aci...