Transcriptional Activities of the Sleeping Beauty Transposon and Shielding Its Genetic Cargo With Insulators

• Published in: Molecular therapy Vol. 16; no. 2; pp. 359 - 369
• Main Authors: Walisko, Oliver, Schorn, Andrea, Rolfs, Frank, Devaraj, Anantharam, Miskey, Csaba, Izsvák, Zsuzsanna, Ivics, Zoltán
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2008; Elsevier Limited
• Subjects: 5' Untranslated Regions - genetics; Binding sites; Chromatin Immunoprecipitation; Gene expression; Gene therapy; Genomes; HeLa Cells; HMGB2 Protein - genetics; HMGB2 Protein - metabolism; Humans; Immunoprecipitation; Leukemia; Models, Genetic; Promoter Regions, Genetic - genetics; Protein Binding; Retroelements - genetics; T-Lymphocytes - metabolism; Transcription, Genetic; Transposases - genetics; Transposases - metabolism; Two-Hybrid System Techniques; Vectors (Biology); Viruses; Berlin Germany; Germany

The Sleeping Beauty (SB) transposable element shows efficient transposition in human cells, and provides long-term transgene expression in preclinical animal models. Random chromosomal insertion of SB vectors represents a safety issue in human gene therapeutic applications, due to potential genotoxi...