Ethnoveterinary study for antidermatophytic activity of Piper betle, Alpinia galanga and Allium ascalonicum extracts in vitro
Crude ethanolic extracts of Piper betle leaves (Piperaceae), Alpinia galanga rhizomes (Zingiberaceae) and Allium ascalonicum bulbs (Liliaceae) were tested against selected zoonotic dermatophytes ( Microsporum canis, Microsporum gypseum and Trichophyton mentagrophyte) and the yeast-like Candida albic...
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Published in | Research in veterinary science Vol. 84; no. 1; pp. 80 - 84 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier India Pvt Ltd
01.02.2008
Elsevier Limited |
Subjects | |
Online Access | Get full text |
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Summary: | Crude ethanolic extracts of
Piper betle leaves (Piperaceae),
Alpinia galanga rhizomes (Zingiberaceae) and
Allium ascalonicum bulbs (Liliaceae) were tested against selected zoonotic dermatophytes (
Microsporum canis,
Microsporum gypseum and
Trichophyton mentagrophyte) and the yeast-like
Candida albicans. A broth dilution method was employed to determine the inhibitory effect of the extracts and compared to those of ketoconazole and griseofulvin. All extracts suppressed the growth of the fungi in a concentration-dependent manner. Among the extracts tested,
P. betle exhibited more effective antifungal properties with average IC
50 values ranging from 110.44 to 119.00
μg/ml. Subsequently, 10%
Piper betle (Pb) cream was formulated, subjected to physical and microbial limit test and evaluated for antifungal effect. The disc diffusion assay revealed comparable zones of inhibition between discs of Pb cream containing 80
μg
P. betle extract and 80
μg ketoconazole against tested fungi at 96
h after incubation. Thereafter, the inhibitory effect of Pb cream markedly decreased and completely lost effectiveness by day 7. In summary, the results supported the traditional wisdom of herbal remedy use and suggested a potential value-addition to agricultural products. It was suggested that the Pb cream has potential therapeutic value for treatment of dermatophytosis. However, clinical testing as well as improving the Pb cream formulation with greater efficacy and duration of action would be of interest and awaits further investigation. |
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Bibliography: | http://dx.doi.org/10.1016/j.rvsc.2007.03.006 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0034-5288 1532-2661 |
DOI: | 10.1016/j.rvsc.2007.03.006 |