Chondrocyte-specific genomic editing enabled by hybrid exosomes for osteoarthritis treatment

• Published in: Theranostics Vol. 12; no. 11; pp. 4866 - 4878
• Main Authors: Liang, Yujie, Xu, Xiao, Xu, Limei, Iqbal, Zoya, Ouyang, Kan, Zhang, Huawei, Wen, Chunyi, Duan, Li, Xia, Jiang
• Format: Journal Article
• Language: English
• Published: Australia Ivyspring International Publisher Pty Ltd 01.01.2022; Ivyspring International Publisher
• Subjects: Animals; Arthritis; Cartilage; Cartilage, Articular - metabolism; Chondrocytes - metabolism; CRISPR; Dendritic cells; Disease; Exosomes - genetics; Exosomes - metabolism; Extracellular matrix; Gene Editing; Genetic engineering; Genome editing; Genomes; Genomics; Liposomes - metabolism; Matrix Metalloproteinase 13 - genetics; Matrix Metalloproteinase 13 - metabolism; Osteoarthritis; Osteoarthritis - metabolism; Peptides; Plasmids; Proteins; Rats; Research Paper; MMP-13; hybrid exosome; CRISPR/Cas9; Therapeutic genome editing; osteoarthritis; cartilage

A cell-specific delivery vehicle is required to achieve gene editing of the disease-associated cells, so the hereditable genome editing reactions are confined within these cells without affecting healthy cells. A hybrid exosome-based nano-sized delivery vehicle derived by fusion of engineered exosom...