Promising bactericidal approach of dihydrazone analogues against bio-film forming Gram-negative bacteria and molecular mechanistic studies
Gram-negative members of the ESCAPE family are more difficult to treat, due to the presence of an additional barrier in the form of a lipopolysaccharide layer and the efficiency of efflux pumps to pump out the drugs from the cytoplasm. The development of alternative therapeutic strategies to tackle...
Saved in:
Published in | RSC advances Vol. 8; no. 10; pp. 5473 - 5483 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Royal Society of Chemistry
01.01.2018
The Royal Society of Chemistry |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Gram-negative members of the ESCAPE family are more difficult to treat, due to the presence of an additional barrier in the form of a lipopolysaccharide layer and the efficiency of efflux pumps to pump out the drugs from the cytoplasm. The development of alternative therapeutic strategies to tackle ESCAPE Gram-negative members is of extreme necessity to provide a solution to the cause of life-threatening infections. The present investigations demonstrated that compounds 17, 20, 25 and 26 possessing the presence of electron donating (OH and OCH
) groups on the phenyl rings are highly potent; whereas compounds 9, 10, 15, 16, 18, 33 and 36 showed moderate activity against Gram-negative bacteria. An excellent dose-dependent antibacterial activity was established compared to that of the standard antibiotic ampicillin. Significant anti-biofilm properties were measured quantitatively, showing optical density (O.D) values of 0.51 ± 015, 0.63 ± 0.20, 0.38 ± 0.07 and 0.62 ± 0.11 at 492 nm and the leakage of cellular components by the compounds, such as 17, 20, 25 and 26, increased the O.D. of respective treated samples compared to the control. In addition, the implication of experimental results is discussed in the light of the lack of survivability of planktonic bacteria and biofilm destruction
. These results revealed the great significance of the development of a new generation of synthetic materials with greater efficacy in anti-biofilm properties by targeting to lock the bio-film associated protein Bap in Gram-negative bacteria. |
---|---|
AbstractList | Gram-negative members of the ESCAPE family are more difficult to treat, due to the presence of an additional barrier in the form of a lipopolysaccharide layer and the efficiency of efflux pumps to pump out the drugs from the cytoplasm. The development of alternative therapeutic strategies to tackle ESCAPE Gram-negative members is of extreme necessity to provide a solution to the cause of life-threatening infections. The present investigations demonstrated that compounds 17, 20, 25 and 26 possessing the presence of electron donating (OH and OCH
) groups on the phenyl rings are highly potent; whereas compounds 9, 10, 15, 16, 18, 33 and 36 showed moderate activity against Gram-negative bacteria. An excellent dose-dependent antibacterial activity was established compared to that of the standard antibiotic ampicillin. Significant anti-biofilm properties were measured quantitatively, showing optical density (O.D) values of 0.51 ± 015, 0.63 ± 0.20, 0.38 ± 0.07 and 0.62 ± 0.11 at 492 nm and the leakage of cellular components by the compounds, such as 17, 20, 25 and 26, increased the O.D. of respective treated samples compared to the control. In addition, the implication of experimental results is discussed in the light of the lack of survivability of planktonic bacteria and biofilm destruction
. These results revealed the great significance of the development of a new generation of synthetic materials with greater efficacy in anti-biofilm properties by targeting to lock the bio-film associated protein Bap in Gram-negative bacteria. Gram-negative members of the ESCAPE family are more difficult to treat, due to the presence of an additional barrier in the form of a lipopolysaccharide layer and the efficiency of efflux pumps to pump out the drugs from the cytoplasm. The development of alternative therapeutic strategies to tackle ESCAPE Gram-negative members is of extreme necessity to provide a solution to the cause of life-threatening infections. The present investigations demonstrated that compounds 17 , 20 , 25 and 26 possessing the presence of electron donating (OH and OCH 3 ) groups on the phenyl rings are highly potent; whereas compounds 9 , 10 , 15 , 16 , 18 , 33 and 36 showed moderate activity against Gram-negative bacteria. An excellent dose-dependent antibacterial activity was established compared to that of the standard antibiotic ampicillin. Significant anti-biofilm properties were measured quantitatively, showing optical density (O.D) values of 0.51 ± 015, 0.63 ± 0.20, 0.38 ± 0.07 and 0.62 ± 0.11 at 492 nm and the leakage of cellular components by the compounds, such as 17 , 20 , 25 and 26 , increased the O.D. of respective treated samples compared to the control. In addition, the implication of experimental results is discussed in the light of the lack of survivability of planktonic bacteria and biofilm destruction in vitro . These results revealed the great significance of the development of a new generation of synthetic materials with greater efficacy in anti-biofilm properties by targeting to lock the bio-film associated protein Bap in Gram-negative bacteria. Gram-negative members of the ESCAPE family are more difficult to treat, due to the presence of an additional barrier in the form of a lipopolysaccharide layer and the efficiency of efflux pumps to pump out the drugs from the cytoplasm. The development of alternative therapeutic strategies to tackle ESCAPE Gram-negative members is of extreme necessity to provide a solution to the cause of life-threatening infections. The present investigations demonstrated that compounds 17, 20, 25 and 26 possessing the presence of electron donating (OH and OCH 3 ) groups on the phenyl rings are highly potent; whereas compounds 9, 10, 15, 16, 18, 33 and 36 showed moderate activity against Gram-negative bacteria. An excellent dose-dependent antibacterial activity was established compared to that of the standard antibiotic ampicillin. Significant anti-biofilm properties were measured quantitatively, showing optical density (O.D) values of 0.51 ± 015, 0.63 ± 0.20, 0.38 ± 0.07 and 0.62 ± 0.11 at 492 nm and the leakage of cellular components by the compounds, such as 17, 20, 25 and 26, increased the O.D. of respective treated samples compared to the control. In addition, the implication of experimental results is discussed in the light of the lack of survivability of planktonic bacteria and biofilm destruction in vitro . These results revealed the great significance of the development of a new generation of synthetic materials with greater efficacy in anti-biofilm properties by targeting to lock the bio-film associated protein Bap in Gram-negative bacteria. Gram-negative members of the ESCAPE family are more difficult to treat, due to the presence of an additional barrier in the form of a lipopolysaccharide layer and the efficiency of efflux pumps to pump out the drugs from the cytoplasm. Gram-negative members of the ESCAPE family are more difficult to treat, due to the presence of an additional barrier in the form of a lipopolysaccharide layer and the efficiency of efflux pumps to pump out the drugs from the cytoplasm. The development of alternative therapeutic strategies to tackle ESCAPE Gram-negative members is of extreme necessity to provide a solution to the cause of life-threatening infections. The present investigations demonstrated that compounds 17, 20, 25 and 26 possessing the presence of electron donating (OH and OCH3) groups on the phenyl rings are highly potent; whereas compounds 9, 10, 15, 16, 18, 33 and 36 showed moderate activity against Gram-negative bacteria. An excellent dose-dependent antibacterial activity was established compared to that of the standard antibiotic ampicillin. Significant anti-biofilm properties were measured quantitatively, showing optical density (O.D) values of 0.51 ± 015, 0.63 ± 0.20, 0.38 ± 0.07 and 0.62 ± 0.11 at 492 nm and the leakage of cellular components by the compounds, such as 17, 20, 25 and 26, increased the O.D. of respective treated samples compared to the control. In addition, the implication of experimental results is discussed in the light of the lack of survivability of planktonic bacteria and biofilm destruction in vitro. These results revealed the great significance of the development of a new generation of synthetic materials with greater efficacy in anti-biofilm properties by targeting to lock the bio-film associated protein Bap in Gram-negative bacteria. |
Author | Sridhara, M B Shantharam, C S Manukumar, H M Vivek, H K Bukhari, S N A Qin, Hua-Li Rakesh, K P |
Author_xml | – sequence: 1 givenname: K P orcidid: 0000-0001-9317-8462 surname: Rakesh fullname: Rakesh, K P email: qinhuali@whut.edu.cn organization: Department of Pharmaceutical Engineering, School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology 205 Luoshi Road Wuhan 430073 PR China qinhuali@whut.edu.cn +86 27 87749300 – sequence: 2 givenname: H K orcidid: 0000-0002-5808-5583 surname: Vivek fullname: Vivek, H K organization: Analytical Research and Development, Syngene International Ltd Biocon Park, Bommasandra Industrial Estate Bangaluru-560099 Karnataka India – sequence: 3 givenname: H M orcidid: 0000-0002-6488-7136 surname: Manukumar fullname: Manukumar, H M organization: Department of Studies in Biotechnology, University of Mysore Manasagangotri Mysuru-570006 Karnataka India – sequence: 4 givenname: C S orcidid: 0000-0002-1021-0584 surname: Shantharam fullname: Shantharam, C S organization: Department of Chemistry, Pooja Bhagavath Memorial Mahajana Education Centre Mysuru-570016 Karnataka India – sequence: 5 givenname: S N A orcidid: 0000-0001-8125-7972 surname: Bukhari fullname: Bukhari, S N A email: qinhuali@whut.edu.cn organization: Department of Pharmaceutical Engineering, School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology 205 Luoshi Road Wuhan 430073 PR China qinhuali@whut.edu.cn +86 27 87749300 – sequence: 6 givenname: Hua-Li surname: Qin fullname: Qin, Hua-Li email: qinhuali@whut.edu.cn organization: Department of Pharmaceutical Engineering, School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology 205 Luoshi Road Wuhan 430073 PR China qinhuali@whut.edu.cn +86 27 87749300 – sequence: 7 givenname: M B surname: Sridhara fullname: Sridhara, M B email: sridhara.mb@gmail.com organization: Department of Chemistry, Rani Channamma University Vidyasangama Belagavi-591156 Karnataka India sridhara.mb@gmail.com +91 9663983459 |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/35542417$$D View this record in MEDLINE/PubMed |
BookMark | eNpdkdtqFTEUhoNUbN32xgeQgDdFGM1hJpncCGWjW6GgiF6HNTnMTplJtslMoT6CT21qD1RzswL51r9W_v85OoopOoReUvKWEq7eGZmBciHo-ASdMNKKhhGhjh7dj9FpKZekHtFRJugzdMy7rmUtlSfo99ec5lBCHPEAZnE5mGBhwnA45ARmj5PHNuyvbYZfdTCGCFMaV1cwjBBiWfAQUuPDNGOf8nyjs8swN9GNsIQrd68KtdPiOU3OrBNkPDuzhxjKEgwuy2qDKy_QUw9Tcad3dYN-fPzwffupufiy-7w9v2hMS8TSiF60XDlgtPOgvJGks4xy1Zre0U5RxrinrPey_tAIwb2zreyHgVjHrWx7vkHvb3UP6zA7a1xcMkz6kMMM-VonCPrflxj2ekxXWhHZU8KqwNmdQE4_qxWLrg4aN00QXVqLZkKwrlWSdhV9_R96mdZcPawUoaRXRNQUN-jNLWVyKiU7_7AMJfomZb2V387_pryr8KvH6z-g95nyP0Lspt4 |
CitedBy_id | crossref_primary_10_1039_C8RA02903B crossref_primary_10_1016_j_ejmech_2020_113134 crossref_primary_10_1016_j_micpath_2020_104499 crossref_primary_10_1016_j_molstruc_2021_130953 crossref_primary_10_1016_j_ejmcr_2021_100015 crossref_primary_10_1016_j_ejmech_2018_11_031 crossref_primary_10_1016_j_ejmech_2023_116072 crossref_primary_10_1155_2024_9941516 crossref_primary_10_1016_j_procbio_2023_10_024 crossref_primary_10_1038_s41598_019_44304_1 crossref_primary_10_1039_C9RA03380G crossref_primary_10_1016_j_bioorg_2020_103885 crossref_primary_10_1016_j_bioorg_2020_104316 crossref_primary_10_1016_j_micpath_2019_03_002 crossref_primary_10_1093_jambio_lxae104 crossref_primary_10_1007_s11164_020_04359_6 crossref_primary_10_1016_j_ejmech_2020_112832 crossref_primary_10_1016_j_micpath_2018_07_025 crossref_primary_10_1002_ardp_202100266 crossref_primary_10_1016_j_ejmech_2021_113442 crossref_primary_10_1016_j_ijbiomac_2021_01_190 crossref_primary_10_1186_s13065_023_00958_7 crossref_primary_10_1016_j_bioorg_2019_103093 crossref_primary_10_1016_j_ejmech_2020_113069 crossref_primary_10_2174_1871520620666200225104558 crossref_primary_10_1016_j_ejmech_2020_112892 crossref_primary_10_1016_j_rechem_2023_101151 crossref_primary_10_1016_j_bioorg_2019_103133 crossref_primary_10_1039_D0RA05319H crossref_primary_10_1016_j_biopha_2022_113302 crossref_primary_10_2174_1570180819666220317151208 crossref_primary_10_1016_j_rechem_2020_100067 crossref_primary_10_3390_pr12061055 crossref_primary_10_1016_j_bmcl_2019_02_003 crossref_primary_10_1002_ardp_202000223 crossref_primary_10_1016_j_bioorg_2021_105116 crossref_primary_10_1016_j_cdc_2019_100193 crossref_primary_10_1007_s00044_020_02549_w crossref_primary_10_1016_j_ejmech_2020_112245 crossref_primary_10_1016_j_biocel_2019_105566 crossref_primary_10_1002_ardp_202000165 |
Cites_doi | 10.4161/viru.2.5.17724 10.1039/C7MD00486A 10.1016/j.arabjc.2017.09.017 10.1016/j.bmcl.2017.05.032 10.1016/j.bioorg.2016.07.001 10.1128/CMR.15.2.167-193.2002 10.3390/medicines4020025 10.1016/S1473-3099(11)70316-4 10.1016/j.foodcont.2011.11.030 10.1186/s13613-015-0061-0 10.1039/C6RA23374K 10.1515/hc-2013-0035 10.1038/nrmicro2319 10.1038/srep18877 10.1039/C7MD00111H 10.3390/medicina47030019 10.1086/595011 10.1038/ja.2014.142 10.1016/j.bmcl.2015.01.010 10.1039/C7MD00209B 10.1111/j.1469-0691.2011.03570.x 10.1016/j.addr.2005.04.002 10.1586/eri.13.12 10.1016/S0140-6736(01)05321-1 10.1016/j.jconrel.2011.07.002 10.1038/nrmicro3155 10.1007/s00044-017-1878-x 10.1038/s41598-017-11597-z 10.1038/nrmicro2415 |
ContentType | Journal Article |
Copyright | This journal is © The Royal Society of Chemistry. Copyright Royal Society of Chemistry 2018 This journal is © The Royal Society of Chemistry 2018 The Royal Society of Chemistry |
Copyright_xml | – notice: This journal is © The Royal Society of Chemistry. – notice: Copyright Royal Society of Chemistry 2018 – notice: This journal is © The Royal Society of Chemistry 2018 The Royal Society of Chemistry |
DBID | NPM AAYXX CITATION 7SR 8BQ 8FD JG9 7X8 5PM |
DOI | 10.1039/c7ra13661g |
DatabaseName | PubMed CrossRef Engineered Materials Abstracts METADEX Technology Research Database Materials Research Database MEDLINE - Academic PubMed Central (Full Participant titles) |
DatabaseTitle | PubMed CrossRef Materials Research Database Engineered Materials Abstracts Technology Research Database METADEX MEDLINE - Academic |
DatabaseTitleList | PubMed CrossRef Materials Research Database |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Chemistry |
EISSN | 2046-2069 |
EndPage | 5483 |
ExternalDocumentID | 10_1039_C7RA13661G 35542417 |
Genre | Journal Article |
GrantInformation_xml | – fundername: ; grantid: Unassigned |
GroupedDBID | -JG 0-7 0R~ 53G AAFWJ AAHBH AAIWI AAJAE AARTK AAWGC AAXHV ABEMK ABGFH ABPDG ABXOH ACGFS ADBBV ADMRA AEFDR AENEX AESAV AFLYV AFPKN AFVBQ AGEGJ AGRSR AGSTE AHGCF AKBGW ALMA_UNASSIGNED_HOLDINGS ANUXI APEMP ASKNT AUDPV BCNDV BLAPV BSQNT C6K EBS EE0 EF- EJD GROUPED_DOAJ H13 HZ~ H~N J3I M~E NPM O9- OK1 PGMZT R7C R7G RCNCU RPM RPMJG RRC RSCEA RVUXY SLH SMJ ZCN AAEMU AAYXX ABASK AETIL ANBJS AUNWK CITATION ECGLT J3G J3H RAOCF ROYLF YAE 7SR 8BQ 8FD JG9 7X8 5PM |
ID | FETCH-LOGICAL-c406t-686439ea215fa9fc705d21394c8e1591223f128f7424c663fed478bb0de3d7483 |
IEDL.DBID | RPM |
ISSN | 2046-2069 |
IngestDate | Tue Sep 17 21:21:39 EDT 2024 Sat Aug 17 00:53:45 EDT 2024 Fri Sep 13 07:24:43 EDT 2024 Fri Aug 23 02:35:47 EDT 2024 Sat Sep 28 08:21:17 EDT 2024 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 10 |
Language | English |
License | This journal is © The Royal Society of Chemistry. |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c406t-686439ea215fa9fc705d21394c8e1591223f128f7424c663fed478bb0de3d7483 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ORCID | 0000-0002-5808-5583 0000-0001-8125-7972 0000-0002-1021-0584 0000-0002-6488-7136 0000-0001-9317-8462 |
OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078102/ |
PMID | 35542417 |
PQID | 2010890610 |
PQPubID | 2047525 |
PageCount | 11 |
ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_9078102 proquest_miscellaneous_2662549715 proquest_journals_2010890610 crossref_primary_10_1039_C7RA13661G pubmed_primary_35542417 |
PublicationCentury | 2000 |
PublicationDate | 2018-01-01 |
PublicationDateYYYYMMDD | 2018-01-01 |
PublicationDate_xml | – month: 01 year: 2018 text: 2018-01-01 day: 01 |
PublicationDecade | 2010 |
PublicationPlace | England |
PublicationPlace_xml | – name: England – name: Cambridge |
PublicationTitle | RSC advances |
PublicationTitleAlternate | RSC Adv |
PublicationYear | 2018 |
Publisher | Royal Society of Chemistry The Royal Society of Chemistry |
Publisher_xml | – name: Royal Society of Chemistry – name: The Royal Society of Chemistry |
References | Lewis (C7RA13661G-(cit13)/*[position()=1]) 2005; 70 Mace (C7RA13661G-(cit27)/*[position()=1]) 2017; 4 Manukumar (C7RA13661G-(cit22)/*[position()=1]) 2017; 7 Mohammad (C7RA13661G-(cit1)/*[position()=1]) 2015; 68 Huh (C7RA13661G-(cit26)/*[position()=1]) 2011; 156 Flemming (C7RA13661G-(cit12)/*[position()=1]) 2010; 8 Da Silva Meira (C7RA13661G-(cit29)/*[position()=1]) 2012; 25 Rakesh (C7RA13661G-(cit14)/*[position()=1]) 2015; 25 Giedraitienė (C7RA13661G-(cit7)/*[position()=1]) 2011; 47 Wang (C7RA13661G-(cit18)/*[position()=1]) 2017; 8 Rakesh (C7RA13661G-(cit15)/*[position()=1]) 2016; 6 Rakesh (C7RA13661G-(cit17)/*[position()=1]) 2017; 26 Rakesh (C7RA13661G-(cit20)/*[position()=1]) 2015; 6 Rakesh (C7RA13661G-(cit16)/*[position()=1]) 2016; 68 Wilson (C7RA13661G-(cit8)/*[position()=1]) 2014; 12 Zha (C7RA13661G-(cit31)/*[position()=1]) 2017; 27 Stewart (C7RA13661G-(cit10)/*[position()=1]) 2001; 358 Donlan (C7RA13661G-(cit11)/*[position()=1]) 2002; 15 Mu (C7RA13661G-(cit30)/*[position()=1]) 2016; 6 Akbar (C7RA13661G-(cit21)/*[position()=1]) 2013; 19 Piddock (C7RA13661G-(cit3)/*[position()=1]) 2012; 12 Manukumar (C7RA13661G-(cit23)/*[position()=1]) 2017 Manukumar (C7RA13661G-(cit24)/*[position()=1]) 2017; 8 Pendleton (C7RA13661G-(cit6)/*[position()=1]) 2013; 11 Chen (C7RA13661G-(cit19)/*[position()=1]) 2017; 8 Ruppe (C7RA13661G-(cit4)/*[position()=1]) 2015; 5 Magiorakos (C7RA13661G-(cit2)/*[position()=1]) 2012; 18 Boucher (C7RA13661G-(cit5)/*[position()=1]) 2009; 48 Wright (C7RA13661G-(cit9)/*[position()=1]) 2005; 57 Archer (C7RA13661G-(cit28)/*[position()=1]) 2011; 2 Andersson (C7RA13661G-(cit25)/*[position()=1]) 2010; 8 |
References_xml | – volume: 2 start-page: 445 year: 2011 ident: C7RA13661G-(cit28)/*[position()=1] publication-title: Virulence doi: 10.4161/viru.2.5.17724 contributor: fullname: Archer – volume: 70 start-page: 267 year: 2005 ident: C7RA13661G-(cit13)/*[position()=1] publication-title: Biochemistry contributor: fullname: Lewis – volume: 8 start-page: 2181 issue: 12 year: 2017 ident: C7RA13661G-(cit24)/*[position()=1] publication-title: MedChemComm doi: 10.1039/C7MD00486A contributor: fullname: Manukumar – year: 2017 ident: C7RA13661G-(cit23)/*[position()=1] publication-title: Arabian J. Chem. doi: 10.1016/j.arabjc.2017.09.017 contributor: fullname: Manukumar – volume: 27 start-page: 3148 year: 2017 ident: C7RA13661G-(cit31)/*[position()=1] publication-title: Bioorg. Med. Chem. Lett. doi: 10.1016/j.bmcl.2017.05.032 contributor: fullname: Zha – volume: 68 start-page: 1 year: 2016 ident: C7RA13661G-(cit16)/*[position()=1] publication-title: Bioorg. Chem. doi: 10.1016/j.bioorg.2016.07.001 contributor: fullname: Rakesh – volume: 15 start-page: 167 year: 2002 ident: C7RA13661G-(cit11)/*[position()=1] publication-title: Clin. Microbiol. Rev. doi: 10.1128/CMR.15.2.167-193.2002 contributor: fullname: Donlan – volume: 4 start-page: 25 year: 2017 ident: C7RA13661G-(cit27)/*[position()=1] publication-title: Medicines doi: 10.3390/medicines4020025 contributor: fullname: Mace – volume: 12 start-page: 249 year: 2012 ident: C7RA13661G-(cit3)/*[position()=1] publication-title: Lancet Infect. Dis. doi: 10.1016/S1473-3099(11)70316-4 contributor: fullname: Piddock – volume: 25 start-page: 469 year: 2012 ident: C7RA13661G-(cit29)/*[position()=1] publication-title: Food Control doi: 10.1016/j.foodcont.2011.11.030 contributor: fullname: Da Silva Meira – volume: 5 start-page: 21 year: 2015 ident: C7RA13661G-(cit4)/*[position()=1] publication-title: Ann. Intensive Care doi: 10.1186/s13613-015-0061-0 contributor: fullname: Ruppe – volume: 6 start-page: 108315 year: 2016 ident: C7RA13661G-(cit15)/*[position()=1] publication-title: RSC Adv. doi: 10.1039/C6RA23374K contributor: fullname: Rakesh – volume: 19 start-page: 265 year: 2013 ident: C7RA13661G-(cit21)/*[position()=1] publication-title: Heterocycl. Commun. doi: 10.1515/hc-2013-0035 contributor: fullname: Akbar – volume: 8 start-page: 260 year: 2010 ident: C7RA13661G-(cit25)/*[position()=1] publication-title: Nat. Rev. Microbiol. doi: 10.1038/nrmicro2319 contributor: fullname: Andersson – volume: 6 start-page: 18877 year: 2016 ident: C7RA13661G-(cit30)/*[position()=1] publication-title: Sci. Rep. doi: 10.1038/srep18877 contributor: fullname: Mu – volume: 8 start-page: 1173 year: 2017 ident: C7RA13661G-(cit18)/*[position()=1] publication-title: MedChemComm doi: 10.1039/C7MD00111H contributor: fullname: Wang – volume: 47 start-page: 137 year: 2011 ident: C7RA13661G-(cit7)/*[position()=1] publication-title: Medicina doi: 10.3390/medicina47030019 contributor: fullname: Giedraitienė – volume: 48 start-page: 1 year: 2009 ident: C7RA13661G-(cit5)/*[position()=1] publication-title: Clin. Infect. Dis. doi: 10.1086/595011 contributor: fullname: Boucher – volume: 6 start-page: 254 year: 2015 ident: C7RA13661G-(cit20)/*[position()=1] publication-title: Eurasian J. Anal. Chem contributor: fullname: Rakesh – volume: 68 start-page: 259 year: 2015 ident: C7RA13661G-(cit1)/*[position()=1] publication-title: J. Antibiot. doi: 10.1038/ja.2014.142 contributor: fullname: Mohammad – volume: 25 start-page: 1072 year: 2015 ident: C7RA13661G-(cit14)/*[position()=1] publication-title: Bioorg. Med. Chem. Lett. doi: 10.1016/j.bmcl.2015.01.010 contributor: fullname: Rakesh – volume: 8 start-page: 1706 year: 2017 ident: C7RA13661G-(cit19)/*[position()=1] publication-title: MedChemComm doi: 10.1039/C7MD00209B contributor: fullname: Chen – volume: 18 start-page: 268 year: 2012 ident: C7RA13661G-(cit2)/*[position()=1] publication-title: Clin. Microbiol. Infect. doi: 10.1111/j.1469-0691.2011.03570.x contributor: fullname: Magiorakos – volume: 57 start-page: 1451 year: 2005 ident: C7RA13661G-(cit9)/*[position()=1] publication-title: Adv. Drug Delivery Rev. doi: 10.1016/j.addr.2005.04.002 contributor: fullname: Wright – volume: 11 start-page: 297 year: 2013 ident: C7RA13661G-(cit6)/*[position()=1] publication-title: Expert Rev. Anti-Infect. Ther. doi: 10.1586/eri.13.12 contributor: fullname: Pendleton – volume: 358 start-page: 135 year: 2001 ident: C7RA13661G-(cit10)/*[position()=1] publication-title: Lancet doi: 10.1016/S0140-6736(01)05321-1 contributor: fullname: Stewart – volume: 156 start-page: 128 year: 2011 ident: C7RA13661G-(cit26)/*[position()=1] publication-title: J. Controlled Release doi: 10.1016/j.jconrel.2011.07.002 contributor: fullname: Huh – volume: 12 start-page: 35 year: 2014 ident: C7RA13661G-(cit8)/*[position()=1] publication-title: Nat. Rev. Microbiol. doi: 10.1038/nrmicro3155 contributor: fullname: Wilson – volume: 26 start-page: 1675 year: 2017 ident: C7RA13661G-(cit17)/*[position()=1] publication-title: Med. Chem. Res. doi: 10.1007/s00044-017-1878-x contributor: fullname: Rakesh – volume: 7 start-page: 11414 year: 2017 ident: C7RA13661G-(cit22)/*[position()=1] publication-title: Sci. Rep. doi: 10.1038/s41598-017-11597-z contributor: fullname: Manukumar – volume: 8 start-page: 623 year: 2010 ident: C7RA13661G-(cit12)/*[position()=1] publication-title: Nat. Rev. Microbiol. doi: 10.1038/nrmicro2415 contributor: fullname: Flemming |
SSID | ssj0000651261 |
Score | 2.440714 |
Snippet | Gram-negative members of the ESCAPE family are more difficult to treat, due to the presence of an additional barrier in the form of a lipopolysaccharide layer... |
SourceID | pubmedcentral proquest crossref pubmed |
SourceType | Open Access Repository Aggregation Database Index Database |
StartPage | 5473 |
SubjectTerms | Ampicillin Antibiotics Bacteria Biofilms Chemistry Cytoplasm Efflux Gram-negative bacteria Optical density Optical properties Survivability |
Title | Promising bactericidal approach of dihydrazone analogues against bio-film forming Gram-negative bacteria and molecular mechanistic studies |
URI | https://www.ncbi.nlm.nih.gov/pubmed/35542417 https://www.proquest.com/docview/2010890610/abstract/ https://search.proquest.com/docview/2662549715 https://pubmed.ncbi.nlm.nih.gov/PMC9078102 |
Volume | 8 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1NT9wwEB0BB8qlaukHoRS5aq9mnXUS20e0KqBKVKgqErfIjp0lEklQdjmUn8CvZuysV2x76y2WP2J5nLw39vgZ4FvOuNAqYzRTltOsqKdUMWuodJxjgkmV-4PClz-Li-vsx01-swV5PAsTgvYr05x0d-1J19yG2Mr7tprEOLHJ1eVMeYUaNp1sw7bg_IWLPv5-EcOKNEqRcjWpxKBTjkA034Ndj6-IWmITh_4hl3_HSL4AnbM38HrFFsnp2Ku3sOW6fXg1i5e0vYOnq6HHZ8QfYkbd5aqxWCNKhZO-Jra5_WMH_dh3juhuXKxZED3XDVJDYpqe1s1dSzx79e2cD7qlnZsHRfDYqsaalrTxLl3SOn9iOIg8k8UYivgers--_55d0NX1CrRCFF_SQno24jSCfq1VXQmW2ykSwqySDklOisShRvSq0XnOKiQmtbOZkMYw67gVmeQfYKfDrh8AcZw5nRsZ9PS4kSoTRtRTZO6F1jItEvgaB7u8H1U0yrD7zVU5E79Og3XOEziKdihXX9Ki9Lv1UiHrYAl8WWfjyPqNDd25_gHLFIX3c0WaJ_BxNNv6NdHeCYgNg64LeH3tzRycdkFnezXNDv-75ifYw-7LccXmCHaWw4P7jBxmaY6D738cZu4ze571vQ |
link.rule.ids | 230,315,733,786,790,870,891,27957,27958,53827,53829 |
linkProvider | National Library of Medicine |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3PT9swFH5iTBpc2G8IY5un7Zo2iZPYPqJq0G0UoQkmbpEdOyUaSVDaHuBP4K_ec1JXlJ22WyLbiZ1n5_ue_fwZ4EsSUCZFHPix0NSP0yLyRaCVzw2leBNwkdiNwpPTdHwRf79MLjcgcXthuqD9XJWD-roa1OVVF1t5U-VDFyc2PJuMhFWoCaLhE3iK4zViD5z0_geMKJaGToyUimHOWhlShKLpNjyzCIu4xdaR6C96-ThK8gHsHD2HX67CfbTJ78Firgb53SMtx39u0QvYWRJRctgnv4QNU7-CrZE7_-013J-1DV4jtBHVSzrnpcYSToWcNAXR5dWtbuVdUxsi634eaEbkVJbIOokqG78orytiibF9znErK782005s3D1VYklNKndML6mM3Yzc6UeTWR_l-AYujr6ej8b-8uQGP0eCMPdTbomOkcgnCimKnAWJjpBrxjk3yJ9C5CQFAmOBfnmcI-cpjI4ZVyrQhmoWc_oWNmus-h4QQwMjE8U7qT6quIiZYkWETkEqJQ9TDz47K2Y3vUBH1i2sU5GN2M_DzuzHHhw4A2fLQTrLbCAAF0hoAg8-rZLxy9o1E1mbZoF50tS60CxMPNjt-8PqNa4jecDWesoqg5XuXk9B-3cS3kt77_93yY-wNT6fnGQn305_vINtbArvJ4YOYHPeLsx7pEpz9aEbGH8ApCoWyg |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB5BkUovvB-BAkZwzebhJLaP1cK2PFqtEJUqLpEd29uIJllldw_0J_CrGSeb1W659ZYotmNnxplv7PE3AB_TkDIpktBPhKZ-ktnYF6FWPjeU4k3IReoOCp-eZSfnydeL9GIr1VcXtF-oclRfVaO6vOxiK-dVEQxxYsH0dCwcQ00YB3Ntg7twD-dsLLYc9f4njJYsiwZCUiqCgrUyomiOZgew76ws2i62a43-g5g3IyW3TM_kIfwaOt1HnPwerZZqVFzf4HO81agewYM1ICVHfZHHcMfUT-D-eMgD9xT-TtsGr9HEEdVTOxelxhoDGzlpLNHl5R_dyuumNkTW_XrQgsiZLBF9ElU2vi2vKuIAsmvnuJWVX5tZRzo-tCqxpibVkK6XVMYdSu54pMmij3Z8BueTzz_HJ_46g4NfIFBY-hl3gMdIxBVWCluwMNUxYs6k4AZxVITYxKKBtOifJwViH2t0wrhSoTZUs4TT57BXY9dfAjE0NDJVvKPso4qLhClmY3QOMil5lHnwYZBkPu-JOvJug52KfMx-HHWiP_bgcBByvp6si9wFBHCBwCb04P3mMX5Zt3cia9OssEyWOVeaRakHL3qd2LxmUCYP2I62bAo4Cu_dJ6gDHZX3Wuavbl3zHexPP03y71_Ovr2GAxwJ79eHDmFv2a7MG0RMS_W2mxv_AEVVGUo |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Promising+bactericidal+approach+of+dihydrazone+analogues+against+bio-film+forming+Gram-negative+bacteria+and+molecular+mechanistic+studies&rft.jtitle=RSC+advances&rft.au=Rakesh%2C+K+P&rft.au=Vivek%2C+H+K&rft.au=Manukumar%2C+H+M&rft.au=Shantharam%2C+C+S&rft.date=2018-01-01&rft.eissn=2046-2069&rft.volume=8&rft.issue=10&rft.spage=5473&rft_id=info:doi/10.1039%2Fc7ra13661g&rft_id=info%3Apmid%2F35542417&rft.externalDocID=35542417 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2046-2069&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2046-2069&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2046-2069&client=summon |