ZO-1 interactions with F-actin and occludin direct epithelial polarization and single lumen specification in 3D culture

• Published in: Journal of cell science Vol. 130; no. 1; pp. 243 - 259
• Main Authors: Odenwald, Matthew A, Choi, Wangsun, Buckley, Aaron, Shashikanth, Nitesh, Joseph, Nora E, Wang, Yitang, Warren, Michael H, Buschmann, Mary M, Pavlyuk, Roman, Hildebrand, Jeffrey, Margolis, Ben, Fanning, Alan S, Turner, Jerrold R
• Format: Journal Article
• Language: English
• Published: England The Company of Biologists Ltd 01.01.2017
• Subjects: Actin; Actins - metabolism; alpha Catenin - metabolism; Auditory defects; Binding; Cell culture; Cell Culture Techniques - methods; Cell Line; Cell Polarity; Cell Proliferation; Clonal deletion; Cues; Cysts; Deletion mutant; Epithelial Cells - cytology; Epithelial Cells - metabolism; Extracellular matrix; Gene Knockdown Techniques; Humans; Lumens; Mitosis; Morphogenesis; Mutants; Occludin - metabolism; Organs; Phenotype; Polarization; Protein Binding; Protein Transport; Proteins; Scaffolding; Structural proteins; Tight Junctions - metabolism; Zonula occludens-1 protein; Zonula Occludens-1 Protein - chemistry; Zonula Occludens-1 Protein - metabolism; α-Catenin; Morphogenesis; Cell polarity; Epithelial Cells; Tight junctions; Lumen Formation

Epithelia within tubular organs form and expand lumens. Failure of these processes can result in serious developmental anomalies. Although tight junction assembly is crucial to epithelial polarization, the contribution of specific tight junction proteins to lumenogenesis is undefined. Here, we show...