Serine hydroxymethyltransferase participates in the synthesis of cysteine-rich storage proteins in rice seed

• Published in: Plant science (Limerick) Vol. 312; p. 111049
• Main Authors: Matsusaka, Hiroaki, Fukuda, Masako, Elakhdar, Ammar, Kumamaru, Toshihiro
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.11.2021
• Subjects: Genetic linkage map; Rice; SHMT; Storage protein; Storage protein; SHMT; Genetic linkage map; Rice
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/j.plantsci.2021.111049

•lcrp mutation influences the synthesis of cysteine-rich storage proteins.•The LCRP gene encodes a serine hydroxymethyltransferase (SHMT).•lcrp mutation influences PB formation. The low level of cysteine-rich proteins (lcrp) mutation indicates a decrease in cysteine-rich (CysR) prolamines, α-globulin, and glutelin. To identify the causing factor of lcrp mutation, to elucidate its function, and to elucidate the role of CysR proteins in the formation of protein bodies (PBs), lcrp mutant was analyzed. A linkage map of the LCRP gene was constructed and genomic DNA sequencing of a predicted gene within the mapped region demonstrated that LCRP encodes a serine hydroxymethyltransferase, which participates in glycine-serine interconversion of one-carbon metabolism in the sulfur assimilation pathway. The levels of l-Ser, Gly, and Met in the sulfur assimilation pathway in the lcrp seeds increased significantly compared to that in the wildtype (WT). As the lcrp mutation influences the growth of shoot and root, the effects of the addition to the medium of amino acids and other compounds on the sulfur assimilation pathway were studied. Electron-lucent PBs surrounded by ribosome-attached membranes were observed accumulating cysteine-poor prolamines in the lcrp seeds. Additionally, glutelin-containing PBs were smaller and distorted in the lcrp seeds compared to those in the WT. These analyses of PBs in the lcrp seeds suggest that cysteine-rich proteins play an important role in the formation of PBs in rice.